Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode | |
| Meng, Ling2; Wu, Ping2; Chen, Guoxiang1; Cai, Chenxin2; Sun, Yongming3; Yuan, Zhenhong3 | |
| 2009-02-15 | |
| 发表期刊 | BIOSENSORS & BIOELECTRONICS
 |
| ISSN | 0956-5663 |
| 卷号 | 24期号:6页码:1751-1756 |
| 摘要 | A glutamate biosensor based on the electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide (NADH), which was generated by the enzymatic reaction, was developed via employing a single-walled carbon nanotubes/thionine (Th-SWNTs) nanocomposite as a mediator and an enzyme immobilization matrix. The biosensor, which was fabricated by immobilizing glutamate dehydrogenase (GIDH) on the surface of Th-SWNTs, exhibited a rapid response (ca. 5 s), a low detection limit (0.1 mu M), a wide and useful linear range (0.5-400 mu M), high sensitivity (137.3 +/- 15.7) mu A mM(-1) cm(-2), higher biological affinity, as well as good stability and repeatability. In addition, the common interfering species, such as ascorbic acid, uric acid, and 4-acetamidophenol, did not cause any interference due to the use of a low operating potential (190 mV vs. NHE). The biosensor can be used to quantify the concentration of glutamate in the physiological level. The Th-SWNTs system represents a simple and effective approach to the integration of dehydrogenase and electrodes, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications including biosensors and biofuel cells. (C) 2008 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 其他摘要 | A glutamate biosensor based on the electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide (NADH), which was generated by the enzymatic reaction, was developed via employing a single-walled carbon nanotubes/thionine (Th-SWNTs) nanocomposite as a mediator and an enzyme immobilization matrix. The biosensor, which was fabricated by immobilizing glutamate dehydrogenase (GIDH) on the surface of Th-SWNTs, exhibited a rapid response (ca. 5 s), a low detection limit (0.1 mu M), a wide and useful linear range (0.5-400 mu M), high sensitivity (137.3 +/- 15.7) mu A mM(-1) cm(-2), higher biological affinity, as well as good stability and repeatability. In addition, the common interfering species, such as ascorbic acid, uric acid, and 4-acetamidophenol, did not cause any interference due to the use of a low operating potential (190 mV vs. NHE). The biosensor can be used to quantify the concentration of glutamate in the physiological level. The Th-SWNTs system represents a simple and effective approach to the integration of dehydrogenase and electrodes, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications including biosensors and biofuel cells. |
| 关键词 | Biosensor Dehydrogenase Coenzyme Glutamate Carbon Nanotubes |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.bios.2008.09.001 |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| URL | 查看原文 |
| 关键词[WOS] | AMPEROMETRIC BIOSENSOR ; DEHYDROGENASE ; ENZYME ; OXIDASE ; GLUCOSE ; BRAIN ; PROBES ; SENSOR ; IONS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | National Natural Science Foundation of China (20673057, 20773067);the Program for New Century Excellent Talents in University (NET-06-0508), and the "863" Project (2007AA05Z154) |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| WOS记录号 | WOS:000263778000035 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.giec.ac.cn/handle/344007/3448 |
| 专题 | 中国科学院广州能源研究所 |
| 作者单位 | 1.Nanjing Normal Univ, Coll Life Sci, Nanjing 210097, Peoples R China 2.Nanjing Normal Univ, Coll Chem & Environm Sci, Jiangsu Key Lab Biofunct Mat, Nanjing 210097, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, Biomass Energy Ctr, Guangzhou 510640, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Ling,Wu, Ping,Chen, Guoxiang,et al. Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode[J]. BIOSENSORS & BIOELECTRONICS,2009,24(6):1751-1756. |
| APA | Meng, Ling,Wu, Ping,Chen, Guoxiang,Cai, Chenxin,Sun, Yongming,&Yuan, Zhenhong.(2009).Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode.BIOSENSORS & BIOELECTRONICS,24(6),1751-1756. |
| MLA | Meng, Ling,et al."Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode".BIOSENSORS & BIOELECTRONICS 24.6(2009):1751-1756. |
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