Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method

GIEC OpenIR

	Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method
	Zhao, Cheng 2,4,6; Yao, Qing-Song 2,7; Wang, Can 1,2,3,5; Luo, Mu-Tan 2,4; Huang, Chao 1,2,3,5; Chen, Xue-Fang 1,2,3,5; Tian, Lan-Lan 2,4; Huang, Qian-Lin 2,4; Xiong, Lian1,2,3,5 ; Li, Hai-Long 1,2,3,5; Chen, Xin-De1,2,3,5
	2021-02-01
发表期刊	JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN
ISSN	0253-5106
卷号	43 期号:1 页码:80-87
通讯作者	Huang, Chao(huangchao@ms.giec.ac.cn) ; Chen, Xin-De(cxd_cxd@hotmail.com)
摘要	To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R-2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates.
关键词	Microbial lipid Fluorescent method Lipid content Trichosporon dermatis Lignocellulosic hydrolysates
关键词[WOS]	NILE RED ; INTRACELLULAR LIPIDS ; OIL PRODUCTION ; MICROALGAE
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21606229] ; project of Pearl River S&T Nova Program of Guangzhou[201610010014] ; Science and Technology Project of Guangdong Province[2016A010105016] ; Foundation Project of Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development[y809jj1001] ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences[y407r41001]
WOS研究方向	Chemistry
项目资助者	National Natural Science Foundation of China ; project of Pearl River S&T Nova Program of Guangzhou ; Science and Technology Project of Guangdong Province ; Foundation Project of Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000647273100010
出版者	CHEM SOC PAKISTAN
引用统计
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/33102
专题	中国科学院广州能源研究所
通讯作者	Huang, Chao; Chen, Xin-De
作者单位	1.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Energy Convers, R&D Ctr Xuyi Attapulgite Appl Technol, Xuyi 211700, Peoples R China 6.South China Inst Environm Sci, Guangdong Key Lab Water & Air Pollut Control, Minist Ecol & Environm China, Guangzhou 510535, Peoples R China 7.Univ Sci & Technol China, Nano Sci & Technol Inst, Suzhou 215123, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Zhao, Cheng,Yao, Qing-Song,Wang, Can,et al. Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method[J]. JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN,2021,43(1):80-87.
APA	Zhao, Cheng.,Yao, Qing-Song.,Wang, Can.,Luo, Mu-Tan.,Huang, Chao.,...&Chen, Xin-De.(2021).Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method.JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN,43(1),80-87.
MLA	Zhao, Cheng,et al."Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method".JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN 43.1(2021):80-87.