Kinetic study of lipase-catalyzed esterification of furoic acid to methyl-2-furoate

doi:10.1016/j.bej.2020.107587

GIEC OpenIR

	Kinetic study of lipase-catalyzed esterification of furoic acid to methyl-2-furoate
	Zhang, Yu1 ; Di, Xiaohui 1,2; Wang, Wen 1; Song, Miaojia 1,2; Yu, Qiang 1; Wang, Zhongming1 ; Yuan, Zhenhong1 ; Chen, Xiaoyan 1,2; Xu, Huijuan1 ; Guo, Ying1
	2020-09-15
发表期刊	BIOCHEMICAL ENGINEERING JOURNAL
ISSN	1369-703X
卷号	161 页码:8
通讯作者	Yu, Qiang(yuqiang@ms.giec.ac.cn)
摘要	Methyl-2-furoate (MF) is a high-value compound, and can be produced via esterification of furoic acid derived from biobased furfural. Herein, CAL-B and [bmim] [PF6] were the most efficient lipase and organic medium for MF production, respectively. Based on mass action law and reaction reversibility, a mathematical model was developed. Data analysis showed the newly proposed binary regression was apparently more suitable compared to the commonly used unitary regression. The enzymatic promotion factor m, for esterification and hydrolysis was 1.8159 and 0, while the alcohol inhibition factor n(i) was 0 and 0.06436, respectively. Both the activation energy E-a,E-i for esterification and hydrolysis was 84 kJ/mol, and the pre-exponential factor A(i) was 7.1408 x 10(13) and 7.8962 x 10(13), respectively. Kinetics study demonstrated that CAL-B held significantly more dominant esterification activity compared to hydrolysis activity, whereas alcohol just inhibited hydrolysis activity instead of esterification activity, and the relationship between esterification/hydrolysis rate constants k(i) and temperature agreed with Arrhenius equation. The maximum MF yield was 82.5 % at 24 h, and the yield decreased to 75.4 % after 1 re-use of CAL-B, but maintained about 20 % for next 4 cycles. Totally, the developed model and binary regression analysis method is ideal for in-depth examination of lipase-catalyzed kinetics in heterogeneous system.
关键词	Enzymatic kinetics Heterogeneous Fitting Binary regression Reversible second-order reaction
DOI	10.1016/j.bej.2020.107587
关键词[WOS]	OXIDATIVE ESTERIFICATION ; LEVULINIC ACID ; IONIC LIQUIDS ; IMMOBILIZATION ; STABILIZATION ; BIODIESEL ; ENZYMES
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21978288] ; National Natural Science Foundation of China[51876206] ; National Key R&D Program of China[2018YFB1501701] ; National Key R&D Program of China[2019YFC1906602] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081]
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering
项目资助者	National Natural Science Foundation of China ; National Key R&D Program of China ; Municipal Science and Technology Project of Guangzhou (China)
WOS类目	Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS记录号	WOS:000552131300002
出版者	ELSEVIER
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/27518
专题	中国科学院广州能源研究所
通讯作者	Yu, Qiang
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Zhang, Yu,Di, Xiaohui,Wang, Wen,et al. Kinetic study of lipase-catalyzed esterification of furoic acid to methyl-2-furoate[J]. BIOCHEMICAL ENGINEERING JOURNAL,2020,161:8.
APA	Zhang, Yu.,Di, Xiaohui.,Wang, Wen.,Song, Miaojia.,Yu, Qiang.,...&Guo, Ying.(2020).Kinetic study of lipase-catalyzed esterification of furoic acid to methyl-2-furoate.BIOCHEMICAL ENGINEERING JOURNAL,161,8.
MLA	Zhang, Yu,et al."Kinetic study of lipase-catalyzed esterification of furoic acid to methyl-2-furoate".BIOCHEMICAL ENGINEERING JOURNAL 161(2020):8.