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Enhanced Sugars Production with High Conversion Efficiency from Alkali-pretreated Sugarcane Bagasse by Enzymatic Mixtures
Liu, Yunyun1; Cao, Yunqi1; Yu, Qiang2; Xu, Jingliang2; Yuan, Zhenhong2
2020-05-01
发表期刊BIORESOURCES
ISSN1930-2126
卷号15期号:2页码:3839-3849
通讯作者Yu, Qiang(yuqiang@ms.giec.ac.cn)
摘要Complementary enzymes can considerably enhance the hydrolysis effectiveness of cellulase. The influence of hemicellulase supplementation on high solids saccharification of alkali-pretreated sugarcane bagasse was assessed. Hemicellulase addition of 1200 IU/g substrate with cellulase loading of 10 FPU/g substrate achieved high sugars yield with glucose and xylose conversion efficiency of 95.4% and 87.4%, respectively. To further improve the substrate conversion efficiency based on high sugars production, fed-batch hydrolysis was employed with high solids loading of 20% (w/v) to 25% (w/v). After 96 h hydrolysis with 25% solids loading at cellulase and hemicellulase loading of 20 FPU/g and 1200 IU/g substrate, respectively, the obtained highest total sugars was 242 g/L, with glucose and xylose conversion efficiencies of 98.6% and 94.9%, respectively. An increase in substrate digestibility upon supplementation of mixture enzymes with high sugars production can be realized in high solids fed batch system with proper cellulase and hemicellulase synergism.
关键词Complex enzymes High sugars Substrate digestibility Fed-batch hydrolysis High solids loading
DOI10.15376/biores.15.2.3839-3849
关键词[WOS]SOLIDS ; OPTIMIZATION ; HYDROLYSIS ; ENZYMES ; SACCHARIFICATION ; DIGESTIBILITY ; TECHNOLOGIES
收录类别SCI
语种英语
资助项目Program of Natural Science Basic Research Program of Shaanxi[2018JQ5134] ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources[SKLCUSA-b201802] ; CAS Key Laboratory of Renewable Energy, Science and Technology Planning Project of Xi`an[20193039YF027NS027] ; Guangzhou Institute of Energy Conversion[Y907k51001]
WOS研究方向Materials Science
项目资助者Program of Natural Science Basic Research Program of Shaanxi ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources ; CAS Key Laboratory of Renewable Energy, Science and Technology Planning Project of Xi`an ; Guangzhou Institute of Energy Conversion
WOS类目Materials Science, Paper & Wood
WOS记录号WOS:000540457600124
出版者NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/27264
专题中国科学院广州能源研究所
通讯作者Yu, Qiang
作者单位1.Shaanxi Univ Sci & Technol, Coll Mech & Elect Engn, Xian 710021, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
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GB/T 7714
Liu, Yunyun,Cao, Yunqi,Yu, Qiang,et al. Enhanced Sugars Production with High Conversion Efficiency from Alkali-pretreated Sugarcane Bagasse by Enzymatic Mixtures[J]. BIORESOURCES,2020,15(2):3839-3849.
APA Liu, Yunyun,Cao, Yunqi,Yu, Qiang,Xu, Jingliang,&Yuan, Zhenhong.(2020).Enhanced Sugars Production with High Conversion Efficiency from Alkali-pretreated Sugarcane Bagasse by Enzymatic Mixtures.BIORESOURCES,15(2),3839-3849.
MLA Liu, Yunyun,et al."Enhanced Sugars Production with High Conversion Efficiency from Alkali-pretreated Sugarcane Bagasse by Enzymatic Mixtures".BIORESOURCES 15.2(2020):3839-3849.
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