Comparative metabolomic analysis of furfural stress response in Aspergillus terreus

doi:10.1007/s10570-019-02402-3

GIEC OpenIR

	Comparative metabolomic analysis of furfural stress response in Aspergillus terreus
	Zhang, Xiaohuan 1,2,3,4; Zhang, Yu1,2,3 ; Qi, Wei1,2,3 ; Yuan, Zhenhong1,2,3 ; Wang, Zhongming1,2,3
	2019-10-01
发表期刊	CELLULOSE
ISSN	0969-0239
卷号	26 期号:15 页码:8227-8236
通讯作者	Zhang, Yu(zhangyu@ms.giec.ac.cn)
摘要	Pretreatment of lignocellulosic biomass results in the generation of a great number of inhibitors, which seriously hinder microbial growth and fermentation. Furfural, one inhibitor produced, can impede Aspergillus terreus growth and itaconic acid (IA) production. In this study, chemical mutagenesis was employed to improve the tolerance of A. terreus to furfural, with results showing significant improvement in A. terreus mutant strain growth rates, furfural degradation and reduction in IA production. Metabolomic analysis was applied to establish the underlying mechanisms of furfural tolerance in A. terreus. Differences in metabolic pathways were assessed between mutant and parental strains, when exposed to 0.5 g/L furfural, which induces significantly greater toxicity to the parental strain than the mutant strain. Results show that the higher activity of beta-alanine, glutathione and tryptophan metabolism pathways in parental strains can directly affect the cell content of NAD(+), NADP(+)/NADPH and coenzyme A, which protect cells from furfural damage. Additionally, parental strain can protect cells against furfural toxicity by reducing lysine metabolism. This study identifies four metabolic pathways which are related to furfural tolerance in A. terreus.
关键词	Aspergillus terreus Furfural tolerance Metabolomics Itaconic acid Biorefinery
DOI	10.1007/s10570-019-02402-3
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; ITACONIC ACID ; BETA-ALANINE ; LIGNOCELLULOSIC BIOMASS ; LYSINE BIOSYNTHESIS ; ACETIC-ACID ; FERMENTATION ; PRETREATMENT ; TOLERANCE ; REVEALS
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21506215] ; National Natural Science Foundation of China[21811530623] ; Municipal Science & Technology Project of Guangzhou (China)[201804010081]
WOS研究方向	Materials Science ; Polymer Science
项目资助者	National Natural Science Foundation of China ; Municipal Science & Technology Project of Guangzhou (China)
WOS类目	Materials Science, Paper & Wood ; Materials Science, Textiles ; Polymer Science
WOS记录号	WOS:000489757400002
出版者	SPRINGER
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/25844
专题	中国科学院广州能源研究所
通讯作者	Zhang, Yu
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Zhang, Xiaohuan,Zhang, Yu,Qi, Wei,et al. Comparative metabolomic analysis of furfural stress response in Aspergillus terreus[J]. CELLULOSE,2019,26(15):8227-8236.
APA	Zhang, Xiaohuan,Zhang, Yu,Qi, Wei,Yuan, Zhenhong,&Wang, Zhongming.(2019).Comparative metabolomic analysis of furfural stress response in Aspergillus terreus.CELLULOSE,26(15),8227-8236.
MLA	Zhang, Xiaohuan,et al."Comparative metabolomic analysis of furfural stress response in Aspergillus terreus".CELLULOSE 26.15(2019):8227-8236.