Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production

doi:10.1166/jbmb.2019.1909

GIEC OpenIR

	Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production
	Zhang, Yu1,2,3 ; Zhang, Xiaohuan 1,2,3,4; Yu, Qiang 1,2,3; Yuan, Zhenhong1,2,3 ; Chen, Xiaoyan 1,2,3,5
	2019-12-01
发表期刊	JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
ISSN	1556-6560
卷号	13 期号:6 页码:799-805
通讯作者	Yu, Qiang(yuqiang@ms.giec.ac.cn)
摘要	Itaconic acid (IA) is one of the most important biobased chemicals, and is generally produced by the filamentous fungi Aspergillus terreus. In order to degrade the production cost of IA, the low-cost and renewable lignocelluloses could be used for IA production. However, inhibitors including furfural derived from indispensable lignocelluloses pretreatment, can seriously hinder the microbial growth and fermentation, and microbial IA production is also no exception. Compared to Saccharomyces cerevisiae, less is known about the effects of furfural for Aspergillus terreus. Here, a furfural tolerant strain was obtained by chemical mutagenesis with diethyl sulfate (DES), and the highest furfural tolerable concentration was up to 1.3 g/L in solid medium. The growth, glucose consumption and IA production of mutant and parental strain were compared in fermentation media containing different furfural concentration, which found 0.5 g/L furfural is the critical concentration. In addition, the morphology of organelle structure was observed with transmission electron microscope and the cellular sugar content was quantified to further support the changes in cell morphology and the impact of furfural.
关键词	Aspergillus terreus Furfural Tolerance Mutagenesis TEM
DOI	10.1166/jbmb.2019.1909
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; ACETIC-ACID ; ENHANCED TOLERANCE ; FERMENTATION ; XYLOSE ; CELLS
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21506215] ; National Natural Science Foundation of China[51876206] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081]
WOS研究方向	Chemistry ; Energy & Fuels ; Materials Science
项目资助者	National Natural Science Foundation of China ; Municipal Science and Technology Project of Guangzhou (China)
WOS类目	Chemistry, Applied ; Energy & Fuels ; Materials Science, Biomaterials
WOS记录号	WOS:000482706800008
出版者	AMER SCIENTIFIC PUBLISHERS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/25478
专题	中国科学院广州能源研究所
通讯作者	Yu, Qiang
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.SUNY Coll Environm Sci & Forestry, Dept Paper & Bioproc Engn, Syracuse, NY 13210 USA
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Zhang, Yu,Zhang, Xiaohuan,Yu, Qiang,et al. Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2019,13(6):799-805.
APA	Zhang, Yu,Zhang, Xiaohuan,Yu, Qiang,Yuan, Zhenhong,&Chen, Xiaoyan.(2019).Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,13(6),799-805.
MLA	Zhang, Yu,et al."Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 13.6(2019):799-805.