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Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production
Zhang, Yu1,2,3; Zhang, Xiaohuan1,2,3,4; Xiao, Shiyuan1,2,3,4; Qi, Wei1,2,3; Xu, Jingliang5; Yuan, Zhenhong1,2,3; Wang, Zhongming1,2,3
2019-06-01
发表期刊BIOCHEMICAL GENETICS
ISSN0006-2928
卷号57期号:3页码:443-454
通讯作者Qi, Wei(qiwei@ms.giec.ac.cn) ; Xu, Jingliang(xujl@zzu.edu.cn)
摘要3-Methyl-1-butanol (3MB) is a promising biofuel that can be produced from 2-ketoisocaproate via the common l-leucine biosynthesis pathway. Corynebacterium glutamicum was chosen as a host bacterium because of its strong resistance to isobutanol. In the current study, several strategies were designed to overproduce 3MB in C. glutamicum through a non-fermentation pathway. The engineered C. glutamicum mutant was obtained by silencing the pyruvate dehydrogenase gene complex (aceE) and deleting the lactic dehydrogenase gene (ldh), followed by mutagenesis with diethyl sulfate (DES) and selection with Fmoc-3-4-thiazolyl-l-alanine (FTA). The mutant could produce 659 mg/L of 3MB after 12 h of incubation. To facilitate carbon flux to 3MB biosynthesis, the engineered recombinant was also constructed without branched-chain acid aminotransferase (ilvE) activity by deleting the ilvE gene. This recombinant could produce 697 mg/L of 3MB after 12 h of incubation.
关键词3-Methyl-1-butanol L-Leucine Corynebacterium glutanicum Mutation breeding Non-fermentation pathway
DOI10.1007/s10528-019-09906-4
关键词[WOS]AMINO-ACIDS ; BIOSYNTHESIS ; ISOBUTANOL ; PLASMIDS ; CLONING
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[21506215] ; National Natural Science Foundation of China[21811530626] ; Municipal Science and Technology Program of Guangzhou (China)[201804010081]
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
项目资助者National Natural Science Foundation of China ; Municipal Science and Technology Program of Guangzhou (China)
WOS类目Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号WOS:000473825900006
出版者SPRINGER/PLENUM PUBLISHERS
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/25288
专题中国科学院广州能源研究所
通讯作者Qi, Wei; Xu, Jingliang
作者单位1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Zhengzhou Univ, Sch Chem Engn & Energy, Zhengzhou 450001, Henan, Peoples R China
第一作者单位中国科学院广州能源研究所
推荐引用方式
GB/T 7714
Zhang, Yu,Zhang, Xiaohuan,Xiao, Shiyuan,et al. Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production[J]. BIOCHEMICAL GENETICS,2019,57(3):443-454.
APA Zhang, Yu.,Zhang, Xiaohuan.,Xiao, Shiyuan.,Qi, Wei.,Xu, Jingliang.,...&Wang, Zhongming.(2019).Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production.BIOCHEMICAL GENETICS,57(3),443-454.
MLA Zhang, Yu,et al."Engineering Corynebacterium glutamicum Mutants for 3-Methyl-1-butanol Production".BIOCHEMICAL GENETICS 57.3(2019):443-454.
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