Biocatalytic upgrading of levulinic acid to methyl levulinate in green solvents

doi:10.1016/j.procbio.2019.03.024

GIEC OpenIR

	Biocatalytic upgrading of levulinic acid to methyl levulinate in green solvents
	Di, Xiaohui 1,2,3,4; Zhang, Yu1,2,3 ; Fu, Junying 1,2,3; Yu, Qiang 1,2,3; Wang, Zhongming1,2,3 ; Yuan, Zhenhong1,2,3
	2019-06-01
发表期刊	PROCESS BIOCHEMISTRY
ISSN	1359-5113
卷号	81 页码:33-38
通讯作者	Zhang, Yu(zhangyu@ms.giec.ac.cn) ; Yu, Qiang(yuqiang@ms.giec.ac.cn)
摘要	High value-added utilization of biomass-based platform chemicals has attracted increasing attention in recent years. The present study addresses the upgrading of levulinic acid (LA), an important biomass-based platform chemical, to methyl levulinate via a green and efficient enzymatic approach. Various lipases were assessed, with Novozym((R)) 435 displaying the best results for the esterification of LA. Subsequently, the effect of reaction medium on Novozym((R)) 435 catalyzed esterification was also studied. Compared with other reaction media, 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim] [PF6]) showed the greatest enhancement in yield by suppressing hydrolysis activity. The catalysts reusability was also improved by protection from [bmim][PF6] with 43.2% activity remained after 5 cycles, compared to that of 19.1% in 2-Me THF. Reaction temperature exerted an unusual double-peak relationship over methyl levulinate yield because Novozym((R)) 435 can simultaneously catalyze the esterification of LA and hydrolysis of methyl levulinate, each of which has a different optimal reaction temperature: ca. 30 degrees C and ca. 40 degrees C, respectively. When the molar ratio of LA/methanol, and the volume ratio of methanol/[bmim] [PF6] were 1:5 and 1:3, respectively, methyl levulinate was obtained in 93% yield at 30 degrees C after 24 h with 10 mg/mL catalytic loading.
关键词	Enzyme-Catalyzed esterification Levulinic acid Immobilized lipases Double-Peak relationship Ionic liquid
DOI	10.1016/j.procbio.2019.03.024
关键词[WOS]	IONIC LIQUIDS ; ENZYME CATALYSIS ; CONVERSION ; STABILITY ; BIODIESEL ; LIPASES ; ETHER ; HMF
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[21506215] ; National Natural Science Foundation of China[51876206] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
项目资助者	National Natural Science Foundation of China ; Municipal Science and Technology Project of Guangzhou (China)
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS记录号	WOS:000469158700005
出版者	ELSEVIER SCI LTD
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/25128
专题	中国科学院广州能源研究所
通讯作者	Zhang, Yu; Yu, Qiang
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Di, Xiaohui,Zhang, Yu,Fu, Junying,et al. Biocatalytic upgrading of levulinic acid to methyl levulinate in green solvents[J]. PROCESS BIOCHEMISTRY,2019,81:33-38.
APA	Di, Xiaohui,Zhang, Yu,Fu, Junying,Yu, Qiang,Wang, Zhongming,&Yuan, Zhenhong.(2019).Biocatalytic upgrading of levulinic acid to methyl levulinate in green solvents.PROCESS BIOCHEMISTRY,81,33-38.
MLA	Di, Xiaohui,et al."Biocatalytic upgrading of levulinic acid to methyl levulinate in green solvents".PROCESS BIOCHEMISTRY 81(2019):33-38.