Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method | |
Zhao, Cheng1,2,3,4; Luo, Mu-Tan1,2,3,4; Huang, Chao1,2,3,5; Chen, Xue-Fang1,2,3,5; Xiong, Lian1,2,3,5 ; Li, Hai-Long1,2,3,5; Chen, Xin-De1,2,3,5
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| 2019-03-28 | |
| Source Publication | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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| ISSN | 1082-6068 |
| Pages | 9 |
| Corresponding Author | Huang, Chao(huangchao@ms.giec.ac.cn) ; Chen, Xin-De(cxd_cxd@hotmail.com) |
| Abstract | A simple and accurate Nile Red fluorescent method was built to evaluate the lipid content of three different oleaginous yeasts by one standard curve. The staining of cells can be observed clearly by laser scanning confocal microscope, showing that Nile Red can enter into the cells of oleaginous yeasts easily. A series of conditions such as pretreating temperature, cell suspension concentration (OD600), staining time, Nile Red concentration and the type of suspension solvent were learnt systematically to obtain the optimal process parameters for Nile Red staining. After optimization, the fitting curve of Nile Red fluorescent method was established under suitable conditions (pretreating temperature: 50 degrees C, OD600: 1.0; staining time: 5 mins; Nile Red concentration: 1.0 mu g/mL; suspension solvent: PBS) and it had a suitable correlation coefficient (R-2 = 0.95) for lipid content measurement of different oleaginous yeasts. By this study, the possibility of lipid content determination of different oleaginous yeasts by one fitting curve can be proven and this will improve the efficiency of researches related to microbial lipid production. |
| Keyword | Fluorescent method microbial lipid Nile Red oleaginous yeasts |
| DOI | 10.1080/10826068.2019.1587624 |
| WOS Keyword | MICROALGAE ; BIODIESEL ; FEEDSTOCKS ; VARIETY |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[21606229] ; project of Pearl River S&T Nova Program of Guangzhou[201610010014] ; Science and Technology Project of Guangdong Province[2016A010105016] ; Youth Innovation Promotion Association CAS[2015290] ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences[y407r41001] |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| Funding Organization | National Natural Science Foundation of China ; project of Pearl River S&T Nova Program of Guangzhou ; Science and Technology Project of Guangdong Province ; Youth Innovation Promotion Association CAS ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences |
| WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000465953000001 |
| Publisher | TAYLOR & FRANCIS INC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/24856 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Huang, Chao; Chen, Xin-De |
| Affiliation | 1.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Energy Convers, R&D Ctr Xuyi Attapulgite Appl Technol, Xuyi, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhao, Cheng,Luo, Mu-Tan,Huang, Chao,et al. Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2019:9. |
| APA | Zhao, Cheng.,Luo, Mu-Tan.,Huang, Chao.,Chen, Xue-Fang.,Xiong, Lian.,...&Chen, Xin-De.(2019).Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,9. |
| MLA | Zhao, Cheng,et al."Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2019):9. |
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