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Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method
Zhao, Cheng1,2,3,4; Luo, Mu-Tan1,2,3,4; Huang, Chao1,2,3,5; Chen, Xue-Fang1,2,3,5; Xiong, Lian1,2,3,5; Li, Hai-Long1,2,3,5; Chen, Xin-De1,2,3,5
2019-03-28
发表期刊PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
ISSN1082-6068
页码9
通讯作者Huang, Chao(huangchao@ms.giec.ac.cn) ; Chen, Xin-De(cxd_cxd@hotmail.com)
摘要A simple and accurate Nile Red fluorescent method was built to evaluate the lipid content of three different oleaginous yeasts by one standard curve. The staining of cells can be observed clearly by laser scanning confocal microscope, showing that Nile Red can enter into the cells of oleaginous yeasts easily. A series of conditions such as pretreating temperature, cell suspension concentration (OD600), staining time, Nile Red concentration and the type of suspension solvent were learnt systematically to obtain the optimal process parameters for Nile Red staining. After optimization, the fitting curve of Nile Red fluorescent method was established under suitable conditions (pretreating temperature: 50 degrees C, OD600: 1.0; staining time: 5 mins; Nile Red concentration: 1.0 mu g/mL; suspension solvent: PBS) and it had a suitable correlation coefficient (R-2 = 0.95) for lipid content measurement of different oleaginous yeasts. By this study, the possibility of lipid content determination of different oleaginous yeasts by one fitting curve can be proven and this will improve the efficiency of researches related to microbial lipid production.
关键词Fluorescent method microbial lipid Nile Red oleaginous yeasts
DOI10.1080/10826068.2019.1587624
关键词[WOS]MICROALGAE ; BIODIESEL ; FEEDSTOCKS ; VARIETY
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[21606229] ; project of Pearl River S&T Nova Program of Guangzhou[201610010014] ; Science and Technology Project of Guangdong Province[2016A010105016] ; Youth Innovation Promotion Association CAS[2015290] ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences[y407r41001]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
项目资助者National Natural Science Foundation of China ; project of Pearl River S&T Nova Program of Guangzhou ; Science and Technology Project of Guangdong Province ; Youth Innovation Promotion Association CAS ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号WOS:000465953000001
出版者TAYLOR & FRANCIS INC
引用统计
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/24854
通讯作者Huang, Chao; Chen, Xin-De
作者单位1.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China
3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou, Guangdong, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Guangzhou Inst Energy Convers, R&D Ctr Xuyi Attapulgite Appl Technol, Xuyi, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Cheng,Luo, Mu-Tan,Huang, Chao,et al. Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2019:9.
APA Zhao, Cheng.,Luo, Mu-Tan.,Huang, Chao.,Chen, Xue-Fang.,Xiong, Lian.,...&Chen, Xin-De.(2019).Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,9.
MLA Zhao, Cheng,et al."Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2019):9.
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