Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations

doi:10.3390/md17020124

GIEC OpenIR

	Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations
	Li, Tao1 ; Xu, Jin 2; Wu, Houbo 1; Jiang, Peiliang 2; Chen, Zishuo 1; Xiang, Wenzhou 1
	2019-02-01
发表期刊	MARINE DRUGS
ISSN	1660-3397
卷号	17 期号:2 页码:16
通讯作者	Xiang, Wenzhou(xwz@scsio.ac.cn)
摘要	Microalgae of the genus Porphyridium show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture nitrogen concentrations to produce Porphyridium biomass rich in B-PE, LC-PUFAs and EPS. Porphyridium purpureum SCS-02 was cultured in ASW culture medium with low nitrogen supply (LN, 3.5 mM), medium nitrogen supply (MN, 5.9 mM) or high nitrogen supply (HN, 17.6 mM). HN significantly enhanced the accumulation of biomass, intracellular protein, B-PE and eicosapentaenoic acid. LN increased the intracellular carbohydrate and arachidonic acid content, and promoted the secretion of EPS. The total lipids content was almost unaffected by nitrogen concentration. Based on these results, a semi-continuous two-step process was proposed, which included the production of biomass rich in B-PE and LC-PUFAs with sufficient nitrogen, and induced EPS excretion with limited nitrogen and strong light.
关键词	Porphyridium nitrogen phycoerythrin exopolysaccharide polyunsaturated fatty acids
DOI	10.3390/md17020124
关键词[WOS]	CULTURE-MEDIA OPTIMIZATION ; FATTY-ACIDS ; POLYSACCHARIDES PRODUCTION ; EICOSAPENTAENOIC ACID ; UNICELLULAR ALGA ; CRUENTUM ; PHYCOERYTHRIN ; LIPIDS ; EXOPOLYSACCHARIDE ; COPRODUCTION
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31602182] ; PhD Start-up Fund of Natural Science Foundation of Guangdong Province[2016A030310016] ; Science and Technology Planning Project of Fujian Province[2013T3008]
WOS研究方向	Pharmacology & Pharmacy
项目资助者	National Natural Science Foundation of China ; PhD Start-up Fund of Natural Science Foundation of Guangdong Province ; Science and Technology Planning Project of Fujian Province
WOS类目	Chemistry, Medicinal
WOS记录号	WOS:000460795500052
出版者	MDPI
引用统计	被引频次：52[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/24756
专题	中国科学院广州能源研究所
通讯作者	Xiang, Wenzhou
作者单位	1.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,RNAM Ctr Marine, Inst South China Sea Ecol & Environm Engn,South C, Guangzhou 510301, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Li, Tao,Xu, Jin,Wu, Houbo,et al. Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations[J]. MARINE DRUGS,2019,17(2):16.
APA	Li, Tao,Xu, Jin,Wu, Houbo,Jiang, Peiliang,Chen, Zishuo,&Xiang, Wenzhou.(2019).Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations.MARINE DRUGS,17(2),16.
MLA	Li, Tao,et al."Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations".MARINE DRUGS 17.2(2019):16.