Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp.

doi:10.1016/j.renene.2018.01.078

GIEC OpenIR

	Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp.
	Zhang, Yi 1,4; Kong, Xiaoying1,2,3,4 ; Wang, Zhongming1,2,3,4 ; Sun, Yongming1,2,3 ; Zhu, Shunni1,2,3 ; Li, Lianhua1,2,3 ; Lv, Pengmei1,2,3
	2018-09-01
发表期刊	RENEWABLE ENERGY
ISSN	0960-1481
卷号	125 页码:1049-1057
通讯作者	Wang, Zhongming(wangzm@ms.giec.ac.cn) ; Zhu, Shunni(zhusn@ms.giec.ac.cn)
摘要	Cell wall disruption is an essential downstream processing step for improving the efficiency of lipid extraction from microalgae. Enzyme-assisted extraction of lipid from microalga Scenedesmus sp. with cellulase, xylanase and pectinase, using various parameters, such as enzyme concentration, temperature, pH and incubation time, was optimized by central composite design (CCD) coupled with response surface methodology (RSM). Both the lipid extraction from microalgae and the fatty acid methyl esters (FAMES) production under optimal conditions showed a twofold in the yields compared to the control group with no enzymatic treatment. SEM images, FTIR measurement, XPS and HPLC analysis showed that the enzymatic pretreatment caused significant alterations in the cell wall structure of microalgae. And the disruption of microalgal cell walls was primarily attributed to the breakage of beta-glucosidic linkages in cellulose and hemicellulose. The study showed a promising approach can lead to an improvement in the lipid extraction yield from microalgae and further provide valuable information for the use of enzymes in microalgal processes. (C) 2018 Published by Elsevier Ltd.
关键词	Cell wall disruption Enzymatic hydrolysis Lipid extraction yield Microalgae Scenedesmus sp. Optimization Biodiesel
DOI	10.1016/j.renene.2018.01.078
关键词[WOS]	FATTY-ACID-COMPOSITION ; BIODIESEL PRODUCTION ; CELL DISRUPTION ; CHLORELLA-VULGARIS ; REACTION-KINETICS ; MASS-TRANSFER ; WASTE-WATER ; OIL ; TRANSESTERIFICATION ; NANNOCHLOROPSIS
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Guangdong Province[2016A030312007] ; Natural Science Foundation of Guangdong Province[2015A030310032] ; Technical Service Network of the Chinese Academy of Sciences[KFJ-EW-STS-138] ; Science and Technology of Guangdong province[2015B020215011] ; National Science & Technology Pillar Program[2015BAD21B03]
WOS研究方向	Science & Technology - Other Topics ; Energy & Fuels
项目资助者	Natural Science Foundation of Guangdong Province ; Technical Service Network of the Chinese Academy of Sciences ; Science and Technology of Guangdong province ; National Science & Technology Pillar Program
WOS类目	GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY ; Energy & Fuels
WOS记录号	WOS:000433648500093
出版者	PERGAMON-ELSEVIER SCIENCE LTD
引用统计	被引频次：76[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/23603
专题	中国科学院广州能源研究所
通讯作者	Wang, Zhongming; Zhu, Shunni
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Zhang, Yi,Kong, Xiaoying,Wang, Zhongming,et al. Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp.[J]. RENEWABLE ENERGY,2018,125:1049-1057.
APA	Zhang, Yi.,Kong, Xiaoying.,Wang, Zhongming.,Sun, Yongming.,Zhu, Shunni.,...&Lv, Pengmei.(2018).Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp..RENEWABLE ENERGY,125,1049-1057.
MLA	Zhang, Yi,et al."Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp.".RENEWABLE ENERGY 125(2018):1049-1057.