Acclimation of acid-tolerant methanogenic propionate-utilizing culture and microbial community dissecting

doi:10.1016/j.biortech.2017.11.034

GIEC OpenIR

	Acclimation of acid-tolerant methanogenic propionate-utilizing culture and microbial community dissecting
	Li, Ying1 ; Sun, Yongming1,2 ; Li, Lianhua1,3 ; Yuan, Zhenhong1,2,3
	2018-02-01
发表期刊	BIORESOURCE TECHNOLOGY
ISSN	0960-8524
卷号	250 页码:117-123
通讯作者	Li, Lianhua(lilh@ms.giec.ac.cn)
摘要	The acid-tolerant methanogenic propionate degradation culture was acclimated in a propionate-fed semi-continuous bioreactor by daily adjusting the digestate pH. The performance of propionate fermentation, the respond of microbial community structure to the acidic environment, and the microbial network for propionate degradation in the acid-tolerant culture was investigated. The results demonstrated that after long term of acclimation to low pH, the digester could produce methane from propionate at pH 4.8-5.5 with 0.3-0.4 L g(-1) propionic acid (HPr) d(-1) of the volatile solids (VS) methane production. The predominant methanogens shifted from acetoclastic methanogens (similar to 87%) to hydrogenotrophic methanogens (similar to 67%) in the bioreactor with the dropping pH, indicating that hydrogenotrophic methanogens were more acid-tolerant than acetoclastic methanogens. Smithella (similar to 11%), Syntrophobacter (similar to 7%) and Pelotomaculum (similar to 3%) were the main propionate oxidizers in the acid-tolerant propionate-utilizing culture. Methanothrix dominant acetoclastic methanogens, while Methanolinea and Methanospirillum were the major H-2 scavengers to support Syntrophobacter and Pelotomaculum syntrophic propionate degradation.
关键词	Anaerobic digestion Propionate-utilizing culture Acid-tolerant Microbial community
DOI	10.1016/j.biortech.2017.11.034
关键词[WOS]	SULFATE-REDUCING BACTERIUM ; 16S RIBOSOMAL-RNA ; SP-NOV. ; OXIDIZING BACTERIUM ; ANAEROBIC DIGESTERS ; WASTE-WATER ; METHANOSPIRILLUM-HUNGATEI ; SMITHELLA-PROPIONICA ; UASB REACTORS ; FOOD WASTE
收录类别	SCI
语种	英语
资助项目	National Nature Science Foundation of China[51708538] ; Natural Science Foundation of Guangdong Province[2017A030310443] ; High Technology Industrial Science and Technology Plan of Guangdong Province[2013B010204053]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
项目资助者	National Nature Science Foundation of China ; Natural Science Foundation of Guangdong Province ; High Technology Industrial Science and Technology Plan of Guangdong Province
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000430740000015
出版者	ELSEVIER SCI LTD
引用统计	被引频次：63[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/23196
专题	中国科学院广州能源研究所
通讯作者	Li, Lianhua
作者单位	1.Chinese Acad Sci, GuangZhou Inst Energy Convers, Lab Biomass Biochem Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Li, Ying,Sun, Yongming,Li, Lianhua,et al. Acclimation of acid-tolerant methanogenic propionate-utilizing culture and microbial community dissecting[J]. BIORESOURCE TECHNOLOGY,2018,250:117-123.
APA	Li, Ying,Sun, Yongming,Li, Lianhua,&Yuan, Zhenhong.(2018).Acclimation of acid-tolerant methanogenic propionate-utilizing culture and microbial community dissecting.BIORESOURCE TECHNOLOGY,250,117-123.
MLA	Li, Ying,et al."Acclimation of acid-tolerant methanogenic propionate-utilizing culture and microbial community dissecting".BIORESOURCE TECHNOLOGY 250(2018):117-123.