Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
| Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose | |
| Zhu, Chang-hui1,3,4,5; Fang, Zhen2; Su, Tong-chao4; Li, Xing-kang5; Liu, Qi-ying1,3 | |
| 2018-04-01 | |
| Source Publication | CELLULOSE
 |
| ISSN | 0969-0239 |
| Volume | 25Issue:4Pages:2473-2485 |
| Corresponding Author | Fang, Zhen(zhenfang@njau.edu.cn) |
| Abstract | Cellulose-based biochar was prepared via ionothermal carbonization of cellulose in [Bmim]Cl with H(2)SO(4)and subsequent pyrolysis. The biochar was analyzed by a series of characterization methods, indicating that it was a kind of mesoporous carbon suitable for the adsorption of cellulase. Kinetic analysis showed that the immobilized cellulase exhibited higher affinity to carboxymethyl cellulose than free cellulase. The immobilized cellulase, at different pH and temperatures, was more stable than free cellulase. It was used to hydrolyze pretreated cellulose in [Bmim]Cl with a total reducing sugar (TRS) yield of 99.9%. The immobilized cellulase maintained activity of 74.8% after five cycles at an immobilized cellulase/cellulose weight ratio of 30:1. When the cellulose loading was increased by a factor of 5, the TRS yield decreased by only 27.5%. |
| Keyword | Mesoporous biochar Ionothermal carbonization Immobilized cellulase Kinetics analysis Stability |
| DOI | 10.1007/s10570-018-1704-8 |
| WOS Keyword | IONIC LIQUID ; LIGNOCELLULOSIC BIOMASS ; ENZYMATIC-HYDROLYSIS ; CARBON MATERIAL ; GLUCOSE BIOSENSOR ; BETA-GLUCOSIDASE ; NANOPARTICLES ; ENZYMES ; ACID ; STRATEGIES |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Nanjing Agricultural University[68Q-0603] ; National Science Foundation of China[51536009] ; National Science Foundation of China[51576199] |
| WOS Research Area | Materials Science ; Polymer Science |
| Funding Organization | Nanjing Agricultural University ; National Science Foundation of China |
| WOS Subject | Materials Science, Paper & Wood ; Materials Science, Textiles ; Polymer Science |
| WOS ID | WOS:000428925300023 |
| Publisher | SPRINGER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.giec.ac.cn/handle/344007/22977 |
| Collection | 中国科学院广州能源研究所 |
| Corresponding Author | Fang, Zhen |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Nengyuanlu, Guangzhou 510640, Guangdong, Peoples R China 2.Nanjing Agr Univ, Coll Engn, Biomass Grp, 40 Dianjiangtai Rd, Nanjing 210031, Jiangsu, Peoples R China 3.CAS Key Lab Renewable Energy, 2 Nengyuanlu, Guangzhou 510640, Guangdong, Peoples R China 4.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, 88 Xuefulu, Kunming 650223, Yunnan, Peoples R China 5.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| First Author Affilication | GuangZhou Institute of Energy Conversion,Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhu, Chang-hui,Fang, Zhen,Su, Tong-chao,et al. Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose[J]. CELLULOSE,2018,25(4):2473-2485. |
| APA | Zhu, Chang-hui,Fang, Zhen,Su, Tong-chao,Li, Xing-kang,&Liu, Qi-ying.(2018).Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose.CELLULOSE,25(4),2473-2485. |
| MLA | Zhu, Chang-hui,et al."Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose".CELLULOSE 25.4(2018):2473-2485. |
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