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Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva
Shang, Changhua1; Zhu, Shunni1; Qin, Lei1; Alam, Mohammad A.1; Wang, Zhongming1; Yuan, Zhenhong1,2; Xie, Jun3
2017-10-01
Source PublicationJOURNAL OF BIOBASED MATERIALS AND BIOENERGY
ISSN1556-6560
Volume11Issue:5Pages:483-490
Corresponding AuthorYuan, Zhenhong(yuanzh@ms.giec.ac.cn)
AbstractDunaliella parva grew in different nitrogen concentrations, which were associated with lipid accumulation. D. parva could accumulate lipids. However, the underlying mechanism of lipid degradation requires further research. Thus, it is important to analyze lipid degradation to elucidate lipid accumulation. In this report, a gene encoding lipase from the green alga D. parva was isolated and characterized. Experimental results showed that the full-length cDNA of lipase gene was 4,583 bp, with an open reading frame of 3,183 bp, a 73 bp 5'-untranslated region and a 1,327 bp 3'-untranslated region. Potential regulatory elements related to hormones, light and unfavorable environments were found in the 1,828 bp 5'-flanking region. Analysis of lipase gene expression showed a correlation between lipase gene expression and nitrogen concentrations, which indicated that lipase gene may be responsive to nitrogen limitation stress. The cloning and expression analysis of lipase gene provided a basis for studying the regulatory mechanism of lipid degradation and the massive accumulation of lipids or lipase in D. parva.
KeywordDifferential Expression Dunaliella parva Lipase Lipid Degradation Nitrogen Limitation
DOI10.1166/jbmb.2017.1691
WOS KeywordLED LIGHT WAVELENGTHS ; DOMAIN TRIACYLGLYCEROL LIPASE ; INTENSITY SUPPLY STRATEGIES ; MICROALGAE CHLORELLA-SP ; CHLAMYDOMONAS-REINHARDTII ; BIODIESEL PRODUCTION ; NUTRIENT REDUCTION ; BIOGAS UPGRADE ; PURIFICATION ; PERFORMANCE
Indexed BySCI
Language英语
Funding ProjectNatural Science Foundation of Guangdong Province[2016A030313171] ; Natural Science Foundation of Guangdong Province[2016A030312007] ; National Natural Sciences Foundation of China[51476177] ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences[y507j21001]
WOS Research AreaChemistry ; Energy & Fuels ; Materials Science
Funding OrganizationNatural Science Foundation of Guangdong Province ; National Natural Sciences Foundation of China ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences
WOS SubjectChemistry, Applied ; Energy & Fuels ; Materials Science, Biomaterials
WOS IDWOS:000416103100013
PublisherAMER SCIENTIFIC PUBLISHERS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/16776
Collection中国科学院广州能源研究所
Corresponding AuthorYuan, Zhenhong
Affiliation1.Chinese Acad Sci, Key Lab Renewable Energy, Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
2.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China
3.South China Agr Univ, Inst New Energy & New Mat, Key Lab Energy Plants Resource & Utilizat, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Shang, Changhua,Zhu, Shunni,Qin, Lei,et al. Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2017,11(5):483-490.
APA Shang, Changhua.,Zhu, Shunni.,Qin, Lei.,Alam, Mohammad A..,Wang, Zhongming.,...&Xie, Jun.(2017).Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,11(5),483-490.
MLA Shang, Changhua,et al."Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 11.5(2017):483-490.
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