Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva

doi:10.1166/jbmb.2017.1691

GIEC OpenIR

	Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva
	Shang, Changhua1 ; Zhu, Shunni1 ; Qin, Lei1 ; Alam, Mohammad A.1; Wang, Zhongming1 ; Yuan, Zhenhong1,2 ; Xie, Jun 3
	2017-10-01
发表期刊	JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
ISSN	1556-6560
卷号	11 期号:5 页码:483-490
通讯作者	Yuan, Zhenhong(yuanzh@ms.giec.ac.cn)
摘要	Dunaliella parva grew in different nitrogen concentrations, which were associated with lipid accumulation. D. parva could accumulate lipids. However, the underlying mechanism of lipid degradation requires further research. Thus, it is important to analyze lipid degradation to elucidate lipid accumulation. In this report, a gene encoding lipase from the green alga D. parva was isolated and characterized. Experimental results showed that the full-length cDNA of lipase gene was 4,583 bp, with an open reading frame of 3,183 bp, a 73 bp 5'-untranslated region and a 1,327 bp 3'-untranslated region. Potential regulatory elements related to hormones, light and unfavorable environments were found in the 1,828 bp 5'-flanking region. Analysis of lipase gene expression showed a correlation between lipase gene expression and nitrogen concentrations, which indicated that lipase gene may be responsive to nitrogen limitation stress. The cloning and expression analysis of lipase gene provided a basis for studying the regulatory mechanism of lipid degradation and the massive accumulation of lipids or lipase in D. parva.
关键词	Differential Expression Dunaliella parva Lipase Lipid Degradation Nitrogen Limitation
DOI	10.1166/jbmb.2017.1691
关键词[WOS]	LED LIGHT WAVELENGTHS ; DOMAIN TRIACYLGLYCEROL LIPASE ; INTENSITY SUPPLY STRATEGIES ; MICROALGAE CHLORELLA-SP ; CHLAMYDOMONAS-REINHARDTII ; BIODIESEL PRODUCTION ; NUTRIENT REDUCTION ; BIOGAS UPGRADE ; PURIFICATION ; PERFORMANCE
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Guangdong Province[2016A030313171] ; Natural Science Foundation of Guangdong Province[2016A030312007] ; National Natural Sciences Foundation of China[51476177] ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences[y507j21001]
WOS研究方向	Chemistry ; Energy & Fuels ; Materials Science
项目资助者	Natural Science Foundation of Guangdong Province ; National Natural Sciences Foundation of China ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences
WOS类目	Chemistry, Applied ; Energy & Fuels ; Materials Science, Biomaterials
WOS记录号	WOS:000416103100013
出版者	AMER SCIENTIFIC PUBLISHERS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/16776
专题	中国科学院广州能源研究所
通讯作者	Yuan, Zhenhong
作者单位	1.Chinese Acad Sci, Key Lab Renewable Energy, Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China 3.South China Agr Univ, Inst New Energy & New Mat, Key Lab Energy Plants Resource & Utilizat, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Shang, Changhua,Zhu, Shunni,Qin, Lei,et al. Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2017,11(5):483-490.
APA	Shang, Changhua.,Zhu, Shunni.,Qin, Lei.,Alam, Mohammad A..,Wang, Zhongming.,...&Xie, Jun.(2017).Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,11(5),483-490.
MLA	Shang, Changhua,et al."Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 11.5(2017):483-490.