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INDUCTION OF HIGHLY ACTIVE beta-GLUCOSIDASE FROM BIOMASS MATERIALS AND ITS APPLICATION ON LIGNOCELLULOSE HYDROLYSIS AND FERMENTATION
Liang Cuiyi; Xu Huijuan; Zhang Yu; Guo Ying; Chen Xiaoyan; Yuan Zhenhong; He Minchao; Xu Jingliang
2017-07-01
Source PublicationCELLULOSE CHEMISTRY AND TECHNOLOGY
Volume51Issue:7-8Pages:719-729
AbstractTo obtain highly active beta-glucosidase, low value agricultural residues were used to induce enzyme production. The results showed that rice bran was the best substrate to induce beta-glucosidase, likely due to its high magnesium, potassium, and calcium content. The optimum temperature and pH of beta-glucosidase were 65 degrees C and 4.8, respectively. This enzyme was thermo-tolerant and fairly stable, with a long half-life of 15 days at 50 degrees C. In addition, the enzyme activity was best stimulated in a concentration of 10% (v/v) ethanol. The effects of beta-glucosidase on fed-batch hydrolysis of cellulose and high-temperature simultaneous saccharification and fermentation (SSF) were examined. With a cellulase loading of 10 FPU/g of substrate and a beta-glucosidase loading of 15 IU/g of substrate, the final substrate loading of 35% dry mass, sugar concentration could attain 225.84 g/L after 120 h of hydrolysis. When the fed-batch SSF process was performed with Kluyveromyces marxianus NCYC 587 operated at 42 degrees C, after 72 h of fermentation, the maximum ethanol concentration was approximately 49.07 g/L, indicating that beta-glucosidase was suitable for lignocellulose conversion into ethanol.
SubtypeArticle
KeywordBeta-glucosidase Induction Biomass Materials Enzyme Biocatalysis Bioconversion Ethanol Fermentation
WOS HeadingsScience & Technology ; Technology
WOS Subject ExtendedMaterials Science
WOS KeywordSIMULTANEOUS SACCHARIFICATION ; BIOETHANOL PRODUCTION ; KLUYVEROMYCES-MARXIANUS ; TRICHODERMA-REESEI ; ETHANOL-PRODUCTION ; CELLULASE ; PURIFICATION ; IMPROVEMENT ; SELECTION ; RESIDUES
Indexed BySCI
Language英语
Funding OrganizationScience & Technology Project of Guangdong Province(2017 A010105018) ; National High Technology Research and Development Program of China (863 Program)(2015AA020202) ; Bureau of International Cooperation, Chinese Academy of Sciences(182344KYSB20160056) ; Program of National Natural Science Foundation of China(21506215 ; 51606203)
WOS SubjectMaterials Science, Paper & Wood
WOS IDWOS:000413720100014
Citation statistics
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/15860
Collection中国科学院广州能源研究所
AffiliationChinese Acad Sci, Key Lab Renewable Energy, Guangzhou Inst Energy Convers, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Liang Cuiyi,Xu Huijuan,Zhang Yu,et al. INDUCTION OF HIGHLY ACTIVE beta-GLUCOSIDASE FROM BIOMASS MATERIALS AND ITS APPLICATION ON LIGNOCELLULOSE HYDROLYSIS AND FERMENTATION[J]. CELLULOSE CHEMISTRY AND TECHNOLOGY,2017,51(7-8):719-729.
APA Liang Cuiyi.,Xu Huijuan.,Zhang Yu.,Guo Ying.,Chen Xiaoyan.,...&Xu Jingliang.(2017).INDUCTION OF HIGHLY ACTIVE beta-GLUCOSIDASE FROM BIOMASS MATERIALS AND ITS APPLICATION ON LIGNOCELLULOSE HYDROLYSIS AND FERMENTATION.CELLULOSE CHEMISTRY AND TECHNOLOGY,51(7-8),719-729.
MLA Liang Cuiyi,et al."INDUCTION OF HIGHLY ACTIVE beta-GLUCOSIDASE FROM BIOMASS MATERIALS AND ITS APPLICATION ON LIGNOCELLULOSE HYDROLYSIS AND FERMENTATION".CELLULOSE CHEMISTRY AND TECHNOLOGY 51.7-8(2017):719-729.
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