Isolation of Streptomyces sp. Strains Capable of Degrading Lignin Under Alkaline Conditions and Its Degradation Properties

doi:10.1166/jbmb.2016.1627

GIEC OpenIR > 中国科学院广州能源研究所

	Isolation of Streptomyces sp. Strains Capable of Degrading Lignin Under Alkaline Conditions and Its Degradation Properties
	Zhou, Guixiong 1; Zhuang, Xinshu 1; Yuan, Zhenhong 1,2; Tan, Xuesong 1; Qi, Wei 1; Yu, Qiang 1; Wang, Wen 1; Wang, Qiong 1
	2016-12-01
发表期刊	JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
卷号	10 期号:6 页码:458-462
摘要	A bacterial strain (designated as Y-8), capable of efficiently degrading lignin under alkaline conditions, was isolated from saline-alkali soil and identified as Streptomyces sp. based on the morphological and physiological analyses as well as the gene sequences comparison of the 16 S rDNA. The alkali tolerance and lignin degradation capability of the isolated strain were determined. The results indicated that Streptomyces sp. Y-8 achieved the optimum lignin degradation at a temperature of 35 degrees C and a pH value of 9.5 with sucrose as the auxiliary carbon source and ammonium nitrate as the nitrogen source, reaching 32.6% in seven days. In addition, the activity of the three enzymes that were produced from the lignin degradation by Streptomyces sp. Y-8 under alkaline conditions (pH = 9.5), laccase (Lac), lignin peroxidase (Lip), and manganese peroxidase (MnP) was studied. The results indicated that the Lac and Lip activities reached their respective maximums on the fifth and fourth day of lignin degradation, respectively, being 4583.5 U/L, and 1163.2 U/L, respectively, and almost no MnP was detected throughout the process. This study is of great significance to the biological pretreatment of lignocellulose or the treatment of black liquid produced from papermaking under alkaline conditions.
文章类型	Article
关键词	Lignin Biodegradation Alkaline Conditions Enzyme Activity Biological Pretreatment Of Lignocellulose
WOS标题词	Science & Technology ; Physical Sciences ; Technology
DOI	10.1166/jbmb.2016.1627
研究领域[WOS]	Chemistry ; Energy & Fuels ; Materials Science
收录类别	SCI ; ISTP
语种	英语
项目资助者	National Natural Science Foundation of China(21406228 ; Key Projects of International Cooperation and Exchanges NSFC(51561145015) ; 51476179)
WOS类目	Chemistry, Applied ; Energy & Fuels ; Materials Science, Biomaterials
WOS记录号	WOS:000397803900010
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/14078
专题	中国科学院广州能源研究所
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 2.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan Province, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Guixiong,Zhuang, Xinshu,Yuan, Zhenhong,et al. Isolation of Streptomyces sp. Strains Capable of Degrading Lignin Under Alkaline Conditions and Its Degradation Properties[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2016,10(6):458-462.
APA	Zhou, Guixiong.,Zhuang, Xinshu.,Yuan, Zhenhong.,Tan, Xuesong.,Qi, Wei.,...&Wang, Qiong.(2016).Isolation of Streptomyces sp. Strains Capable of Degrading Lignin Under Alkaline Conditions and Its Degradation Properties.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,10(6),458-462.
MLA	Zhou, Guixiong,et al."Isolation of Streptomyces sp. Strains Capable of Degrading Lignin Under Alkaline Conditions and Its Degradation Properties".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 10.6(2016):458-462.