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Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass
Wu, Hongli1; Xue, Yong1; Li, Hailong2; Gan, Lihui1; Liu, Jian1; Long, Minnan1
2017
Source PublicationBIORESOURCES
Volume12Issue:1Pages:434-447
AbstractEfficient utilization of plant biomass by enzymatic hydrolysis is currently studied worldwide but still faces enormous challenges because of the inability to break down lignocellulosic materials with high sugar yields and low enzyme dosage. Therefore, the synergistic action between various enzymes plays an important role in reaching this goal. The synergistic cooperation between a novel acetyl xylan esterase (heterologous expressed at high levels in this study) and four other xylan-degrading enzymes (reported previously) were performed in this study. The acetyl xylan esterase (AnAxe) gene was cloned from Aspergillus niger BE-2 and expressed in Pichia pastoris GS115. The deduced amino acid (aa) sequence consisted of 304-aa and included a 23-aa signal peptide and 281-aa mature protein. The AnAxe was extracellularly expressed with a molecular weight of ca. 31 kDa. The purified AnAxe exhibited maximal specific activity of 480.2 IU/mg at pH 7.0 and 40 degrees C and was still thermostable below 50 degrees C. The metal ions used in this study and EDTA showed a slight effect on the AnAxe. A significant synergistic effect was determined between AnAxe and the other four xylan-degrading enzymes, including endo-j-1,4-xylanases, beta-xylosidases, alpha-L-arabinofurano-sidases, and alpha-glucuronidases, on the degradation of bamboo biomass. The highest degree of synergism was obtained between AnAxe and endo-beta-1,4-xylanases/beta-xylosidases.
SubtypeArticle
KeywordAspergillus Niger Acetyl Xylan Esterase Pichia Pastoris Characterization Synergistic Cooperation
WOS HeadingsScience & Technology ; Technology
DOI10.15376/biores.12.1.434-447
WOS Subject ExtendedMaterials Science
WOS KeywordRESPONSE-SURFACE METHODOLOGY ; ENZYMATIC-HYDROLYSIS ; DEGRADATION ; SYSTEMS ; CLONING ; CHRYSOSPORIUM ; FERMENTATION ; OPTIMIZATION ; PURIFICATION ; XYLOSE
Indexed BySCI
Language英语
Funding OrganizationNatural Science Foundation of Guangdong Province, China(2016A030310124) ; National Natural Science Foundation of China(21303142 ; Xiamen Southern Oceanographic Center(14GZP59HJ29) ; Fujian Provincial Department of Ocean and Fisheries(2015-27) ; Xiamen University(20720150090) ; 31600475)
WOS SubjectMaterials Science, Paper & Wood
WOS IDWOS:000397065200031
Citation statistics
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/14063
Collection中国科学院广州能源研究所
Affiliation1.Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Wu, Hongli,Xue, Yong,Li, Hailong,et al. Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass[J]. BIORESOURCES,2017,12(1):434-447.
APA Wu, Hongli,Xue, Yong,Li, Hailong,Gan, Lihui,Liu, Jian,&Long, Minnan.(2017).Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass.BIORESOURCES,12(1),434-447.
MLA Wu, Hongli,et al."Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass".BIORESOURCES 12.1(2017):434-447.
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