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Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass
Wu, Hongli1; Xue, Yong1; Li, Hailong2; Gan, Lihui1; Liu, Jian1; Long, Minnan1
2017
发表期刊BIORESOURCES
卷号12期号:1页码:434-447
摘要Efficient utilization of plant biomass by enzymatic hydrolysis is currently studied worldwide but still faces enormous challenges because of the inability to break down lignocellulosic materials with high sugar yields and low enzyme dosage. Therefore, the synergistic action between various enzymes plays an important role in reaching this goal. The synergistic cooperation between a novel acetyl xylan esterase (heterologous expressed at high levels in this study) and four other xylan-degrading enzymes (reported previously) were performed in this study. The acetyl xylan esterase (AnAxe) gene was cloned from Aspergillus niger BE-2 and expressed in Pichia pastoris GS115. The deduced amino acid (aa) sequence consisted of 304-aa and included a 23-aa signal peptide and 281-aa mature protein. The AnAxe was extracellularly expressed with a molecular weight of ca. 31 kDa. The purified AnAxe exhibited maximal specific activity of 480.2 IU/mg at pH 7.0 and 40 degrees C and was still thermostable below 50 degrees C. The metal ions used in this study and EDTA showed a slight effect on the AnAxe. A significant synergistic effect was determined between AnAxe and the other four xylan-degrading enzymes, including endo-j-1,4-xylanases, beta-xylosidases, alpha-L-arabinofurano-sidases, and alpha-glucuronidases, on the degradation of bamboo biomass. The highest degree of synergism was obtained between AnAxe and endo-beta-1,4-xylanases/beta-xylosidases.
文章类型Article
关键词Aspergillus Niger Acetyl Xylan Esterase Pichia Pastoris Characterization Synergistic Cooperation
WOS标题词Science & Technology ; Technology
资助者Natural Science Foundation of Guangdong Province, China(2016A030310124) ; National Natural Science Foundation of China(21303142 ; Xiamen Southern Oceanographic Center(14GZP59HJ29) ; Fujian Provincial Department of Ocean and Fisheries(2015-27) ; Xiamen University(20720150090) ; 31600475) ; Natural Science Foundation of Guangdong Province, China(2016A030310124) ; National Natural Science Foundation of China(21303142 ; Xiamen Southern Oceanographic Center(14GZP59HJ29) ; Fujian Provincial Department of Ocean and Fisheries(2015-27) ; Xiamen University(20720150090) ; 31600475)
DOI10.15376/biores.12.1.434-447
研究领域[WOS]Materials Science
关键词[WOS]RESPONSE-SURFACE METHODOLOGY ; ENZYMATIC-HYDROLYSIS ; DEGRADATION ; SYSTEMS ; CLONING ; CHRYSOSPORIUM ; FERMENTATION ; OPTIMIZATION ; PURIFICATION ; XYLOSE
收录类别SCI
语种英语
资助者Natural Science Foundation of Guangdong Province, China(2016A030310124) ; National Natural Science Foundation of China(21303142 ; Xiamen Southern Oceanographic Center(14GZP59HJ29) ; Fujian Provincial Department of Ocean and Fisheries(2015-27) ; Xiamen University(20720150090) ; 31600475) ; Natural Science Foundation of Guangdong Province, China(2016A030310124) ; National Natural Science Foundation of China(21303142 ; Xiamen Southern Oceanographic Center(14GZP59HJ29) ; Fujian Provincial Department of Ocean and Fisheries(2015-27) ; Xiamen University(20720150090) ; 31600475)
WOS类目Materials Science, Paper & Wood
WOS记录号WOS:000397065200031
引用统计
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/14063
专题中国科学院广州能源研究所
作者单位1.Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
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GB/T 7714
Wu, Hongli,Xue, Yong,Li, Hailong,et al. Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass[J]. BIORESOURCES,2017,12(1):434-447.
APA Wu, Hongli,Xue, Yong,Li, Hailong,Gan, Lihui,Liu, Jian,&Long, Minnan.(2017).Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass.BIORESOURCES,12(1),434-447.
MLA Wu, Hongli,et al."Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass".BIORESOURCES 12.1(2017):434-447.
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