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In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media
Zhu, Changhui1,2,4; Guo, Feng1; Guo, Xiaoqian1,3,4; Li, Xingkang4
2016-11-01
发表期刊BIORESOURCES
卷号11期号:4页码:9068-9078
摘要In situ enzymatic saccharification is one promising approach to efficiently convert non-food biomass to glucose as a platform chemical. The goal of the present research was to illustrate the catalytic behaviors and deactivation mechanisms of cellulase and cellobiase in an aqueous-ionic liquid (IL) media. The hydrolysis reaction rate was considerably increased because of the increased porosity and reduced crystallinity of the substrate. To lower the inhibitory effect of accumulated cellobiose on cellulase, a multi-enzyme synergetic system was explored. Compared with the single cellulase saccharification, addition of cellobiase resulted in a substantial increase in total reducing sugars (TRS) yield (88.0% vs. 59.1%) and glucose yield (40.6% vs. 28.9%) when the activity unit ratio of cellobiase/cellulase was 0.95. Cellulase showed a higher stability in aqueous 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) media. However, the activity of cellobiase quickly decreased, by 66.5%, after 6 h of incubation.
文章类型Article
关键词In Situ Saccharification Aqueous-ionic Liquid Media Cellulase Cellobiase Synergism
WOS标题词Science & Technology ; Technology
资助者Chinese Academy of Sciences(Y2XB021K01) ; National Natural Science Foundation of China(31270620) ; Chinese Academy of Sciences(Y2XB021K01) ; National Natural Science Foundation of China(31270620)
DOI10.15376/biores.11.4.9068-9078
研究领域[WOS]Materials Science
关键词[WOS]LIGNOCELLULOSIC BIOMASS ; ENZYMATIC-HYDROLYSIS ; PRETREATMENT ; DISSOLUTION ; SOLVATION ; MECHANISM ; FEATURES
收录类别SCI
语种英语
资助者Chinese Academy of Sciences(Y2XB021K01) ; National Natural Science Foundation of China(31270620) ; Chinese Academy of Sciences(Y2XB021K01) ; National Natural Science Foundation of China(31270620)
WOS类目Materials Science, Paper & Wood
WOS记录号WOS:000391801300071
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/14022
专题中国科学院广州能源研究所
作者单位1.Dalian Univ Technol, Sch Food & Environm, Panjin Campus,2 Dagong Rd, Panjin 124221, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
3.Chinese Acad Sci, Key Lab Trop Plant Resource & Sustainable Use, Xishuangbanna Trop Bot Garden, Plant Funct Genom Lab, 88 Xuefu Rd, Kunming 650223, Peoples R China
4.Chinese Acad Sci, Grad Univ, 19A Yuqun Rd, Beijing 100049, Peoples R China
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GB/T 7714
Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,et al. In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media[J]. BIORESOURCES,2016,11(4):9068-9078.
APA Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,&Li, Xingkang.(2016).In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media.BIORESOURCES,11(4),9068-9078.
MLA Zhu, Changhui,et al."In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media".BIORESOURCES 11.4(2016):9068-9078.
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