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In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media
Zhu, Changhui1,2,4; Guo, Feng1; Guo, Xiaoqian1,3,4; Li, Xingkang4
2016-11-01
Source PublicationBIORESOURCES
ISSN1930-2126
Volume11Issue:4Pages:9068-9078
AbstractIn situ enzymatic saccharification is one promising approach to efficiently convert non-food biomass to glucose as a platform chemical. The goal of the present research was to illustrate the catalytic behaviors and deactivation mechanisms of cellulase and cellobiase in an aqueous-ionic liquid (IL) media. The hydrolysis reaction rate was considerably increased because of the increased porosity and reduced crystallinity of the substrate. To lower the inhibitory effect of accumulated cellobiose on cellulase, a multi-enzyme synergetic system was explored. Compared with the single cellulase saccharification, addition of cellobiase resulted in a substantial increase in total reducing sugars (TRS) yield (88.0% vs. 59.1%) and glucose yield (40.6% vs. 28.9%) when the activity unit ratio of cellobiase/cellulase was 0.95. Cellulase showed a higher stability in aqueous 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) media. However, the activity of cellobiase quickly decreased, by 66.5%, after 6 h of incubation.
SubtypeArticle
KeywordIn Situ Saccharification Aqueous-ionic Liquid Media Cellulase Cellobiase Synergism
WOS HeadingsScience & Technology ; Technology
DOI10.15376/biores.11.4.9068-9078
WOS Subject ExtendedMaterials Science
WOS KeywordLIGNOCELLULOSIC BIOMASS ; ENZYMATIC-HYDROLYSIS ; PRETREATMENT ; DISSOLUTION ; SOLVATION ; MECHANISM ; FEATURES
Indexed BySCI
Language英语
Funding OrganizationChinese Academy of Sciences(Y2XB021K01) ; National Natural Science Foundation of China(31270620)
WOS SubjectMaterials Science, Paper & Wood
WOS IDWOS:000391801300071
PublisherNORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/14022
Collection中国科学院广州能源研究所
Corresponding AuthorGuo, Feng
Affiliation1.Dalian Univ Technol, Sch Food & Environm, Panjin Campus,2 Dagong Rd, Panjin 124221, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
3.Chinese Acad Sci, Key Lab Trop Plant Resource & Sustainable Use, Xishuangbanna Trop Bot Garden, Plant Funct Genom Lab, 88 Xuefu Rd, Kunming 650223, Peoples R China
4.Chinese Acad Sci, Grad Univ, 19A Yuqun Rd, Beijing 100049, Peoples R China
First Author AffilicationGuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,et al. In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media[J]. BIORESOURCES,2016,11(4):9068-9078.
APA Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,&Li, Xingkang.(2016).In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media.BIORESOURCES,11(4),9068-9078.
MLA Zhu, Changhui,et al."In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media".BIORESOURCES 11.4(2016):9068-9078.
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