In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media

doi:10.15376/biores.11.4.9068-9078

GIEC OpenIR > 中国科学院广州能源研究所

	In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media
	Zhu, Changhui 1,2,4; Guo, Feng 1; Guo, Xiaoqian 1,3,4; Li, Xingkang 4
	2016-11-01
发表期刊	BIORESOURCES
ISSN	1930-2126
卷号	11 期号:4 页码:9068-9078
摘要	In situ enzymatic saccharification is one promising approach to efficiently convert non-food biomass to glucose as a platform chemical. The goal of the present research was to illustrate the catalytic behaviors and deactivation mechanisms of cellulase and cellobiase in an aqueous-ionic liquid (IL) media. The hydrolysis reaction rate was considerably increased because of the increased porosity and reduced crystallinity of the substrate. To lower the inhibitory effect of accumulated cellobiose on cellulase, a multi-enzyme synergetic system was explored. Compared with the single cellulase saccharification, addition of cellobiase resulted in a substantial increase in total reducing sugars (TRS) yield (88.0% vs. 59.1%) and glucose yield (40.6% vs. 28.9%) when the activity unit ratio of cellobiase/cellulase was 0.95. Cellulase showed a higher stability in aqueous 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) media. However, the activity of cellobiase quickly decreased, by 66.5%, after 6 h of incubation.
文章类型	Article
关键词	In Situ Saccharification Aqueous-ionic Liquid Media Cellulase Cellobiase Synergism
WOS标题词	Science & Technology ; Technology
DOI	10.15376/biores.11.4.9068-9078
研究领域[WOS]	Materials Science
关键词[WOS]	LIGNOCELLULOSIC BIOMASS ; ENZYMATIC-HYDROLYSIS ; PRETREATMENT ; DISSOLUTION ; SOLVATION ; MECHANISM ; FEATURES
收录类别	SCI
语种	英语
项目资助者	Chinese Academy of Sciences(Y2XB021K01) ; National Natural Science Foundation of China(31270620)
WOS类目	Materials Science, Paper & Wood
WOS记录号	WOS:000391801300071
出版者	NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/14022
专题	中国科学院广州能源研究所
通讯作者	Guo, Feng
作者单位	1.Dalian Univ Technol, Sch Food & Environm, Panjin Campus,2 Dagong Rd, Panjin 124221, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 3.Chinese Acad Sci, Key Lab Trop Plant Resource & Sustainable Use, Xishuangbanna Trop Bot Garden, Plant Funct Genom Lab, 88 Xuefu Rd, Kunming 650223, Peoples R China 4.Chinese Acad Sci, Grad Univ, 19A Yuqun Rd, Beijing 100049, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,et al. In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media[J]. BIORESOURCES,2016,11(4):9068-9078.
APA	Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,&Li, Xingkang.(2016).In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media.BIORESOURCES,11(4),9068-9078.
MLA	Zhu, Changhui,et al."In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media".BIORESOURCES 11.4(2016):9068-9078.