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Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva
Shang, Changhua1,2,3,4; Xu, Xiaoli1,2,3; Yuan, Zhenhong4; Wang, Zhongming4; Hu, Lei5; Alam, Mohammad Asraful4; Xie, Jun1,2,3
2016-08-01
发表期刊JOURNAL OF APPLIED PHYCOLOGY
卷号28期号:4页码:2397-2405
摘要Dunaliella parva can thrive and adapt to salt stress over a wide range of NaCl concentrations which also is related to carotenoid accumulation in Dunaliella. Dunaliella parva can accumulate carotenoids; however, the underlying mechanism of carotenoid accumulation needs further research. Thus, it is necessary to study biosynthesis and regulation of carotenoids for understanding carotenoid accumulation. In the present study, a gene encoding geranylgeranyl diphosphate synthase (GGPS) from the halophilic green alga D. parva has been cloned and analyzed. It is in the branch of terpene metabolism and named as DpGGPS (D. parva GGPS). The full-length complementary DNA (cDNA) of DpGGPS was 1612 bp, including an open reading frame (ORF) of 1059 bp, 189 bp 5'-untranslated region (5'-UTR) and 364 bp 3'-UTR. The 5'-flanking region was obtained by the genome walking method. Potential regulatory elements, associated with hormones, defense, and stress, were found in the 1310 bp 5'-flanking region. Functional characterization of DpGGPS in E. coli BL21(DE3) demonstrated that DpGGPS encoded a functional GGPS. Analysis of DpGGPS expression revealed a correlation between GGPS expression and the shift of NaCl concentration, which indicated that GGPS could be a salt stress responsive gene. Cloning and expression analysis of GGPS provides a foundation for studying the regulatory mechanism of carotenoid biosynthesis, adaptation mechanism to salt stress, and massive accumulation of carotenoids in D. parva.
文章类型Article
关键词Dunaliella Parva Salt Stress Carotenoid Geranylgeranyl Diphosphate Synthase Complementation Analysis Differential Expression
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10811-015-0767-2
研究领域[WOS]Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]BETA-CAROTENE ; FUNCTIONAL-ANALYSIS ; SALINA ; BIOSYNTHESIS ; METABOLISM ; EMPHASIS ; PLANTS ; ALGAE ; LIGHT
收录类别SCI
语种英语
项目资助者National Natural Sciences Foundation of China(51476177) ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences(y507j21001) ; Foundation of Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology (Huaiyin Normal University)(JSBEET1316)
WOS类目Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号WOS:000383571300027
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/13905
专题中国科学院广州能源研究所
作者单位1.South China Agr Univ, Inst New Energy & New Mat, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
2.Minist Agr, Key Lab Energy Plants Resource & Utilizat, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
3.Key Lab Biomass Energy Guangdong Regular Higher E, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
4.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China
5.Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, 111 West Changjiang Rd, Huaian 223300, Jiangsu, Peoples R China
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Shang, Changhua,Xu, Xiaoli,Yuan, Zhenhong,et al. Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(4):2397-2405.
APA Shang, Changhua.,Xu, Xiaoli.,Yuan, Zhenhong.,Wang, Zhongming.,Hu, Lei.,...&Xie, Jun.(2016).Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva.JOURNAL OF APPLIED PHYCOLOGY,28(4),2397-2405.
MLA Shang, Changhua,et al."Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva".JOURNAL OF APPLIED PHYCOLOGY 28.4(2016):2397-2405.
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