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Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva
Shang, Changhua1,2,3,4; Xu, Xiaoli1,2,3; Yuan, Zhenhong4; Wang, Zhongming4; Hu, Lei5; Alam, Mohammad Asraful4; Xie, Jun1,2,3
2016-08-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
Volume28Issue:4Pages:2397-2405
AbstractDunaliella parva can thrive and adapt to salt stress over a wide range of NaCl concentrations which also is related to carotenoid accumulation in Dunaliella. Dunaliella parva can accumulate carotenoids; however, the underlying mechanism of carotenoid accumulation needs further research. Thus, it is necessary to study biosynthesis and regulation of carotenoids for understanding carotenoid accumulation. In the present study, a gene encoding geranylgeranyl diphosphate synthase (GGPS) from the halophilic green alga D. parva has been cloned and analyzed. It is in the branch of terpene metabolism and named as DpGGPS (D. parva GGPS). The full-length complementary DNA (cDNA) of DpGGPS was 1612 bp, including an open reading frame (ORF) of 1059 bp, 189 bp 5'-untranslated region (5'-UTR) and 364 bp 3'-UTR. The 5'-flanking region was obtained by the genome walking method. Potential regulatory elements, associated with hormones, defense, and stress, were found in the 1310 bp 5'-flanking region. Functional characterization of DpGGPS in E. coli BL21(DE3) demonstrated that DpGGPS encoded a functional GGPS. Analysis of DpGGPS expression revealed a correlation between GGPS expression and the shift of NaCl concentration, which indicated that GGPS could be a salt stress responsive gene. Cloning and expression analysis of GGPS provides a foundation for studying the regulatory mechanism of carotenoid biosynthesis, adaptation mechanism to salt stress, and massive accumulation of carotenoids in D. parva.
SubtypeArticle
KeywordDunaliella Parva Salt Stress Carotenoid Geranylgeranyl Diphosphate Synthase Complementation Analysis Differential Expression
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10811-015-0767-2
WOS Subject ExtendedBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS KeywordBETA-CAROTENE ; FUNCTIONAL-ANALYSIS ; SALINA ; BIOSYNTHESIS ; METABOLISM ; EMPHASIS ; PLANTS ; ALGAE ; LIGHT
Indexed BySCI
Language英语
Funding OrganizationNational Natural Sciences Foundation of China(51476177) ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences(y507j21001) ; Foundation of Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology (Huaiyin Normal University)(JSBEET1316)
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000383571300027
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/13905
Collection中国科学院广州能源研究所
Affiliation1.South China Agr Univ, Inst New Energy & New Mat, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
2.Minist Agr, Key Lab Energy Plants Resource & Utilizat, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
3.Key Lab Biomass Energy Guangdong Regular Higher E, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
4.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China
5.Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, 111 West Changjiang Rd, Huaian 223300, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Shang, Changhua,Xu, Xiaoli,Yuan, Zhenhong,et al. Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(4):2397-2405.
APA Shang, Changhua.,Xu, Xiaoli.,Yuan, Zhenhong.,Wang, Zhongming.,Hu, Lei.,...&Xie, Jun.(2016).Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva.JOURNAL OF APPLIED PHYCOLOGY,28(4),2397-2405.
MLA Shang, Changhua,et al."Cloning and differential expression analysis of geranylgeranyl diphosphate synthase gene from Dunaliella parva".JOURNAL OF APPLIED PHYCOLOGY 28.4(2016):2397-2405.
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