Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology

doi:10.1007/s12010-015-1956-9

GIEC OpenIR > 中国科学院广州能源研究所

	Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology
	Huang, Chao 1,2; Wang, Can 1,2; Xiong, Lian1,2 ; Chen, Xue-Fang 1,2; Lin, Xiao-Qing 1,2; Qi, Gao-Xiang 2,3; Shi, Si-Lan 2,3; Wang, Bo 2,3; Chen, Xin-De1,2,4
	2016-04-01
发表期刊	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷号	178 期号:7 页码:1420-1429
摘要	In present study, the beneficial effect of corncob acid hydrolysate environment on lipid fermentation of Trichosporon dermatis was elucidated by method of cell biology (mainly using flow cytometry and microscope) for the first time. Propidium iodide (PI) and rhodamine 123 (Rh123) staining showed that corncob acid hydrolysate environment was favorable for the cell membrane integrity and mitochondrial membrane potential of T. dermatis and thus made its lipid fermentation more efficient. Nile red (NR) staining showed that corncob acid hydrolysate environment made the lipid accumulation of T. dermatis slower, but this influence was not serious. Moreover, the cell morphology of T. dermatis elongated in the corncob acid hydrolysate, but the cell morphology changed as elliptical-like during fermentation. Overall, this work offers one simple and effective method to evaluate the influence of lignocellulosic hydrolysates environment on lipid fermentation.
文章类型	Article
关键词	Corncob Acid Hydrolysate Trichosporon Dermatis Flow Cytometry Microbial Oil Cell Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s12010-015-1956-9
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	NILE RED FLUORESCENCE ; FLOW-CYTOMETRY ; OIL PRODUCTION ; CIDER FERMENTATION ; ESCHERICHIA-COLI ; YEAST ; APOPTOSIS ; ACCUMULATION ; MECHANISMS ; INHIBITORS
收录类别	SCI
语种	英语
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000375614500012
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/11385
专题	中国科学院广州能源研究所
作者单位	1.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China
第一作者单位	中国科学院广州能源研究所
推荐引用方式 GB/T 7714	Huang, Chao,Wang, Can,Xiong, Lian,et al. Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,178(7):1420-1429.
APA	Huang, Chao.,Wang, Can.,Xiong, Lian.,Chen, Xue-Fang.,Lin, Xiao-Qing.,...&Chen, Xin-De.(2016).Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,178(7),1420-1429.
MLA	Huang, Chao,et al."Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 178.7(2016):1420-1429.