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Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region
Wang, Zhiyuan; Lv, Pengmei; Luo, Wen; Yuan, Zhenhong; He, Dong
2016
Source PublicationJOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
Volume62Issue:1Pages:25-30
AbstractA large number of propeptide regions from various proteins have been identified which function as intramolecular chaperones and assist the folding of the respective functional domains. The same polypeptide can fold into an altered conformation because of a mutated intramolecular chaperone and can maintain the "memory" of the folding process (new physicochemical properties). Two new kinds of Rhizomucor miehei lipase (RML) were constructed by replacing its propeptide region with that from either Rhizopus chinensis lipase (RCL) or Rhizopus oryzae lipase (ROL). The enzymatic properties were also analyzed and compared between wild-type RML and the mutants. The results indicated that the same polypeptide can fold into different conformations because of changes in the propeptide region.
SubtypeArticle
KeywordEnzyme Property Intramolecular Chaperone Propeptide Replacing Protein Folding Rhizomucor Miehei Lipases
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.2323/jgam.62.25
WOS Subject ExtendedBiotechnology & Applied Microbiology ; Microbiology
WOS KeywordINTRAMOLECULAR CHAPERONES ; PRO-SEQUENCE ; ENHANCED ACTIVITY ; ESCHERICHIA-COLI ; PRECURSOR ; PROTEASE
Indexed BySCI
Language英语
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000374814700005
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/11276
Collection中国科学院广州能源研究所
AffiliationChinese Acad Sci, Key Lab Renewable Energy & Gas Hydrate, Guangzhou Inst Energy Convers, 2 Nengyuan RD, Guangzhou 510640, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Wang, Zhiyuan,Lv, Pengmei,Luo, Wen,et al. Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region[J]. JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,2016,62(1):25-30.
APA Wang, Zhiyuan,Lv, Pengmei,Luo, Wen,Yuan, Zhenhong,&He, Dong.(2016).Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region.JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,62(1),25-30.
MLA Wang, Zhiyuan,et al."Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region".JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 62.1(2016):25-30.
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