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Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region
Wang, Zhiyuan; Lv, Pengmei; Luo, Wen; Yuan, Zhenhong; He, Dong
2016
发表期刊JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
卷号62期号:1页码:25-30
摘要A large number of propeptide regions from various proteins have been identified which function as intramolecular chaperones and assist the folding of the respective functional domains. The same polypeptide can fold into an altered conformation because of a mutated intramolecular chaperone and can maintain the "memory" of the folding process (new physicochemical properties). Two new kinds of Rhizomucor miehei lipase (RML) were constructed by replacing its propeptide region with that from either Rhizopus chinensis lipase (RCL) or Rhizopus oryzae lipase (ROL). The enzymatic properties were also analyzed and compared between wild-type RML and the mutants. The results indicated that the same polypeptide can fold into different conformations because of changes in the propeptide region.
文章类型Article
关键词Enzyme Property Intramolecular Chaperone Propeptide Replacing Protein Folding Rhizomucor Miehei Lipases
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.2323/jgam.62.25
研究领域[WOS]Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]INTRAMOLECULAR CHAPERONES ; PRO-SEQUENCE ; ENHANCED ACTIVITY ; ESCHERICHIA-COLI ; PRECURSOR ; PROTEASE
收录类别SCI
语种英语
WOS类目Biotechnology & Applied Microbiology ; Microbiology
WOS记录号WOS:000374814700005
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/11276
专题中国科学院广州能源研究所
作者单位Chinese Acad Sci, Key Lab Renewable Energy & Gas Hydrate, Guangzhou Inst Energy Convers, 2 Nengyuan RD, Guangzhou 510640, Guangdong, Peoples R China
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Wang, Zhiyuan,Lv, Pengmei,Luo, Wen,et al. Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region[J]. JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,2016,62(1):25-30.
APA Wang, Zhiyuan,Lv, Pengmei,Luo, Wen,Yuan, Zhenhong,&He, Dong.(2016).Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region.JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,62(1),25-30.
MLA Wang, Zhiyuan,et al."Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region".JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 62.1(2016):25-30.
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