Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region

doi:10.2323/jgam.62.25

GIEC OpenIR > 中国科学院广州能源研究所

	Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region
	Wang, Zhiyuan; Lv, Pengmei; Luo, Wen; Yuan, Zhenhong; He, Dong
	2016
发表期刊	JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
卷号	62 期号:1 页码:25-30
摘要	A large number of propeptide regions from various proteins have been identified which function as intramolecular chaperones and assist the folding of the respective functional domains. The same polypeptide can fold into an altered conformation because of a mutated intramolecular chaperone and can maintain the "memory" of the folding process (new physicochemical properties). Two new kinds of Rhizomucor miehei lipase (RML) were constructed by replacing its propeptide region with that from either Rhizopus chinensis lipase (RCL) or Rhizopus oryzae lipase (ROL). The enzymatic properties were also analyzed and compared between wild-type RML and the mutants. The results indicated that the same polypeptide can fold into different conformations because of changes in the propeptide region.
文章类型	Article
关键词	Enzyme Property Intramolecular Chaperone Propeptide Replacing Protein Folding Rhizomucor Miehei Lipases
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.2323/jgam.62.25
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	INTRAMOLECULAR CHAPERONES ; PRO-SEQUENCE ; ENHANCED ACTIVITY ; ESCHERICHIA-COLI ; PRECURSOR ; PROTEASE
收录类别	SCI
语种	英语
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000374814700005
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.giec.ac.cn/handle/344007/11276
专题	中国科学院广州能源研究所
作者单位	Chinese Acad Sci, Key Lab Renewable Energy & Gas Hydrate, Guangzhou Inst Energy Convers, 2 Nengyuan RD, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zhiyuan,Lv, Pengmei,Luo, Wen,et al. Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region[J]. JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,2016,62(1):25-30.
APA	Wang, Zhiyuan,Lv, Pengmei,Luo, Wen,Yuan, Zhenhong,&He, Dong.(2016).Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region.JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,62(1),25-30.
MLA	Wang, Zhiyuan,et al."Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region".JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 62.1(2016):25-30.