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3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum
Xiao, Shiyuan1,2; Xu, Jingliang1; Chen, Xiaoyan1; Li, Xiekun1,2; Zhang, Yu1; Yuan, Zhenhong1
2016-05-01
发表期刊MOLECULAR BIOTECHNOLOGY
卷号58期号:5页码:311-318
摘要Biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. Higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. In the present work, 3-methyl-1-butanol production in engineered Corynebacterium glutamicum was studied. alpha-Ketoisovalerate decarboxylase gene (kivd) from Lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adhA, and adh3) from three organisms were overexpressed in C. glutamicum. Enzymatic assay and alcohol production results showed that adh3 from Zymomonas mobilis was the optimum candidate for 3-methyl-1-butanol production in C. glutamicum. The recombinant with kivd and adh3 could produce 0.182 g/L of 3-methyl-1-butanol and 0.144 g/L of isobutanol after 12 h of incubation. Further inactivation of the E1 subunit of pyruvate dehydrogenase complex gene (aceE) and lactic dehydrogenase gene (ldh) in the above C. glutamicum strain would improve the 3-Methyl-1-butanol titer to 0.497 g/L after 12 h of incubation.
文章类型Article
关键词3-methyl-1-butanol Corynebacterium Glutamicum Alpha-ketoisovalerate Decarboxylase Alcohol Dehydrogenase Gene Knockout
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12033-016-9929-y
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]PYRUVATE-DEHYDROGENASE COMPLEX ; ESCHERICHIA-COLI ; AMINO-ACIDS ; DECARBOXYLASES ; FERMENTATION ; MUTAGENESIS ; ALCOHOLS ; ENZYME ; GENE
收录类别SCI
语种英语
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号WOS:000374286500001
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/11237
专题中国科学院广州能源研究所
作者单位1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
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Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,et al. 3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum[J]. MOLECULAR BIOTECHNOLOGY,2016,58(5):311-318.
APA Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,Li, Xiekun,Zhang, Yu,&Yuan, Zhenhong.(2016).3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum.MOLECULAR BIOTECHNOLOGY,58(5),311-318.
MLA Xiao, Shiyuan,et al."3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum".MOLECULAR BIOTECHNOLOGY 58.5(2016):311-318.
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