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3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum
Xiao, Shiyuan1,2; Xu, Jingliang1; Chen, Xiaoyan1; Li, Xiekun1,2; Zhang, Yu1; Yuan, Zhenhong1
2016-05-01
Source PublicationMOLECULAR BIOTECHNOLOGY
Volume58Issue:5Pages:311-318
AbstractBiofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. Higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. In the present work, 3-methyl-1-butanol production in engineered Corynebacterium glutamicum was studied. alpha-Ketoisovalerate decarboxylase gene (kivd) from Lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adhA, and adh3) from three organisms were overexpressed in C. glutamicum. Enzymatic assay and alcohol production results showed that adh3 from Zymomonas mobilis was the optimum candidate for 3-methyl-1-butanol production in C. glutamicum. The recombinant with kivd and adh3 could produce 0.182 g/L of 3-methyl-1-butanol and 0.144 g/L of isobutanol after 12 h of incubation. Further inactivation of the E1 subunit of pyruvate dehydrogenase complex gene (aceE) and lactic dehydrogenase gene (ldh) in the above C. glutamicum strain would improve the 3-Methyl-1-butanol titer to 0.497 g/L after 12 h of incubation.
SubtypeArticle
Keyword3-methyl-1-butanol Corynebacterium Glutamicum Alpha-ketoisovalerate Decarboxylase Alcohol Dehydrogenase Gene Knockout
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12033-016-9929-y
WOS Subject ExtendedBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS KeywordPYRUVATE-DEHYDROGENASE COMPLEX ; ESCHERICHIA-COLI ; AMINO-ACIDS ; DECARBOXYLASES ; FERMENTATION ; MUTAGENESIS ; ALCOHOLS ; ENZYME ; GENE
Indexed BySCI
Language英语
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000374286500001
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/11237
Collection中国科学院广州能源研究所
Affiliation1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationGuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,et al. 3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum[J]. MOLECULAR BIOTECHNOLOGY,2016,58(5):311-318.
APA Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,Li, Xiekun,Zhang, Yu,&Yuan, Zhenhong.(2016).3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum.MOLECULAR BIOTECHNOLOGY,58(5),311-318.
MLA Xiao, Shiyuan,et al."3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum".MOLECULAR BIOTECHNOLOGY 58.5(2016):311-318.
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