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Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry
Huang, Chao1,3,4; Zhu, Ding-he2; Wu, Hong1,2; Lou, Wen-yong1; Zong, Min-hua1
2014-03-01
Source PublicationPROCESS BIOCHEMISTRY
Volume49Issue:3Pages:395-401
AbstractThe effect of ten typical organic acids and five aldehydes present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans was evaluated by flow cytometry. Overall, organic acids affected the cell membrane integrity of T. fermentans more significantly than that of aldehydes albeit aldehydes are more toxic to T. fermentans. The PI (Propidium Iodide) uptake rate of T. fermentans' cells gradually decreased as fermentation going on, indicating that T. fermentans could overcome the inhibition of organic acids or aldehydes by adaption. Interestingly, in some cases, the effect of organic acids or aldehydes on the cell membrane integrity of T. fermentans was well related to their hydrophobicity. However, for the outliers, no obvious similar phenomena were observed. Thus, the attack on hydrophobic sites of cell membrane was not the only determinant for the damage of organic acids or aldehydes on cell membrane integrity of T. fermentans. (C) 2013 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordTrichosporon Fermentans Organic Acid Aldehyde Cell Membrane Integrity Flow Cytometry Lignocellulosic Hydrolysate
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2013.12.007
WOS Subject ExtendedBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS KeywordETHANOLOGENIC ESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; LIPID-ACCUMULATION ; OIL PRODUCTION ; ORGANIC-ACIDS ; GROWTH ; CONVERSION ; ALDEHYDES
Indexed BySCI
Language英语
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000334482300006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10708
Collection中国科学院广州能源研究所
Affiliation1.S China Univ Technol, State Key Lab Pulp & Paper Engn, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China
2.S China Univ Technol, Prov Key Lab Green Proc Technol & Prod Safety Nat, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China
3.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
4.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China
First Author AffilicationGuangZhou Institute of Energy Conversion,Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Huang, Chao,Zhu, Ding-he,Wu, Hong,et al. Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry[J]. PROCESS BIOCHEMISTRY,2014,49(3):395-401.
APA Huang, Chao,Zhu, Ding-he,Wu, Hong,Lou, Wen-yong,&Zong, Min-hua.(2014).Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry.PROCESS BIOCHEMISTRY,49(3),395-401.
MLA Huang, Chao,et al."Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry".PROCESS BIOCHEMISTRY 49.3(2014):395-401.
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