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Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum
Ma,Yu-Han1; Wang,Xiang1; Niu,Ying-Fang1; Yang,Zhi-Kai1; Zhang,Meng-Han1; Wang,Zhong-Ming2; Yang,Wei-Dong1; Liu,Jie-Sheng1; Li,Hong-Ye1
2014-08-09
Source PublicationMicrobial Cell Factories
ISSN1475-2859
Volume13Issue:1
Corresponding AuthorLi,Hong-Ye(thyli@jnu.edu.cn)
AbstractAbstractBackgroundMicroalgae have been an emerging biofuel resource; however, the germplasm improvement has been slow due to the lack of molecular tools. Pyruvate dehydrogenase kinase (PDK) deactivates the pyruvate dehydrogenase complex (PDC) which catalyzes the oxidative decarboxylation of pyruvate. Acetyl-CoA production via PDC is important in plant tissues that are active in fatty acid synthesis.ResultsA 1261-bp cDNA of a putative PDK gene (PtPDK) was cloned from a diatom Phaeodactylum tricornutum, and PtPDK antisense knockdown transgenic diatoms were generated. Both PtPDK transcript abundance and enzyme activity were reduced significantly due to antisense knockdown of PtPDK. Neutral lipid content of transgenic diatom cells increased up to 82% as determined by Nile red staining, and fatty acid composition was not altered. Transgenic cells showed slightly lower growth rate but similar cell size with the wild type, hence retaining similar biomass productivity.ConclusionsThis work first obtained a successful engineered diatom regulating a key gene involved in lipid metabolism. Our findings also provide powerful indications in enhancing microalgal lipid production by metabolic engineering for biofuel industry.
KeywordMicroalga Pyruvate dehydrogenase kinase Antisense Lipid Biofuel
DOI10.1186/s12934-014-0100-9
Language英语
WOS IDBMC:10.1186/s12934-014-0100-9
PublisherBioMed Central
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Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10627
Collection中国科学院广州能源研究所
Corresponding AuthorLi,Hong-Ye
Affiliation1.Jinan University; Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes
2.Chinese Academy of Sciences; Guangzhou Institute of Energy Conversion
Recommended Citation
GB/T 7714
Ma,Yu-Han,Wang,Xiang,Niu,Ying-Fang,et al. Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum[J]. Microbial Cell Factories,2014,13(1).
APA Ma,Yu-Han.,Wang,Xiang.,Niu,Ying-Fang.,Yang,Zhi-Kai.,Zhang,Meng-Han.,...&Li,Hong-Ye.(2014).Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum.Microbial Cell Factories,13(1).
MLA Ma,Yu-Han,et al."Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum".Microbial Cell Factories 13.1(2014).
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