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Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass
Zhang, C.; Xing, D.; Xu, J.
2007
Source PublicationANALYTICAL LETTERS
Volume40Issue:9Pages:1672-1685
AbstractWe have described a compact capillary-based continuous-flow polymerase chain reaction ( PCR) microfluidics device, which uses flexible thin film heaters with low thermal mass to construct three isothermal zones. Due to the decreased thermal mass of flexible thin film heater, the low power supply and rapid thermal response was obtained. The energy consumption of a 33-cycle continuous-flow PCR was less than 0.0088 kW h, which is much lower than that of the metal block or liquid bath based capillary continuous-flow PCR microfluidics. Special attention was also paid on the surface passivation of the capillary inner surface based on the competing bovine serum albumin ( BSA), and the results showed the effect of dynamic passivation was superior to that of static passivation. With the help of the dynamic passivation, the 249 bp human beta-actin gene fragment was amplified in 15 min, which is several times faster than that of the conventional PCR machine. In addition, the effect of initial DNA template concentrations on continuous-flow PCR was also investigated.
SubtypeArticle
KeywordContinuous-flow Polymerase Chain Reaction (Pcr) Microfluidics Dna Amplification Thin Film Heater
WOS HeadingsScience & Technology ; Physical Sciences
WOS Subject ExtendedChemistry
WOS KeywordPOLYMERASE-CHAIN-REACTION ; CHIP
Indexed BySCI
Language英语
WOS SubjectChemistry, Analytical
WOS IDWOS:000247761900002
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10275
Collection中国科学院广州能源研究所
Affiliation1.S China Normal Univ, MOE Key Lib Laser Life Sci, Guangzhou 510631, Peoples R China
2.S China Normal Univ, Inst Laser Life Sci, Guangzhou 510631, Peoples R China
3.Chinese Acad Sci, Micro Energy Syst Lab, Guangzhou Inst Energy Convers, Guangzhou, Peoples R China
Recommended Citation
GB/T 7714
Zhang, C.,Xing, D.,Xu, J.. Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass[J]. ANALYTICAL LETTERS,2007,40(9):1672-1685.
APA Zhang, C.,Xing, D.,&Xu, J..(2007).Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass.ANALYTICAL LETTERS,40(9),1672-1685.
MLA Zhang, C.,et al."Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass".ANALYTICAL LETTERS 40.9(2007):1672-1685.
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