Knowledge Management System Of Guangzhou Institute of Energy Conversion, CAS
Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100 | |
Zhang, Yu; Xu, Jing-Liang1; Yuan, Zhen-Hong; Qi, Wei; Liu, Yun-Yun; He, Min-Chao | |
2012-07-01 | |
发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
卷号 | 13期号:7页码:7952-7962 |
摘要 | Two artificial intelligence techniques, namely artificial neural network (ANN) and genetic algorithm (GA) were combined to be used as a tool for optimizing the covalent immobilization of cellulase on a smart polymer, Eudragit L-100. 1-Ethyl-3-(3-dimethyllaminopropyl) carbodiimide (EDC) concentration, N-hydroxysuccinimide (NHS) concentration and coupling time were taken as independent variables, and immobilization efficiency was taken as the response. The data of the central composite design were used to train ANN by back-propagation algorithm, and the result showed that the trained ANN fitted the data accurately (correlation coefficient R-2 = 0.99). Then a maximum immobilization efficiency of 88.76% was searched by genetic algorithm at a EDC concentration of 0.44%, NHS concentration of 0.37% and a coupling time of 2.22 h, where the experimental value was 87.97 +/- 6.45%. The application of ANN based optimization by GA is quite successful. |
文章类型 | Article |
关键词 | Immobilized Enzyme Cellulase Artificial Intelligence Based Optimization Smart Biocatalyst Carbodiimide Coupling |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.3390/ijms13077952 |
研究领域[WOS] | Chemistry |
关键词[WOS] | RESPONSE-SURFACE METHODOLOGY ; NEURAL-NETWORK ; GENETIC ALGORITHM ; HYDROLYSIS ; RSM ; PERFORMANCE ; ADIPATE ; DESIGN ; ANN |
收录类别 | SCI |
语种 | 英语 |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000306239200006 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.giec.ac.cn/handle/344007/10193 |
专题 | 中国科学院广州能源研究所 |
作者单位 | 1.Chinese Acad Sci, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Yu,Xu, Jing-Liang,Yuan, Zhen-Hong,et al. Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(7):7952-7962. |
APA | Zhang, Yu,Xu, Jing-Liang,Yuan, Zhen-Hong,Qi, Wei,Liu, Yun-Yun,&He, Min-Chao.(2012).Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(7),7952-7962. |
MLA | Zhang, Yu,et al."Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.7(2012):7952-7962. |
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