GIEC OpenIR  > 中国科学院广州能源研究所
Engineering Clostridium acetobutylicum for alcohol production
Hou, Xiaohu1,2; Peng, Wanfeng2; Xiong, Lian2; Huang, Chao2; Chen, Xuefang2; Chen, Xinde2; Zhang, Weiguo1
2013-06-20
发表期刊JOURNAL OF BIOTECHNOLOGY
卷号166期号:1-2页码:25-33
摘要While Clostridium acetobutylicum has been used for large-scale butanol production (ABE fermentation), its by-product acetone cannot be used as a biofuel. In this study, C. acetobutylicum was engineered for alcohol titers (butanol plus ethanol). The adc gene was inactivated to eliminate acetone production, and glutathione biosynthetic capability was introduced into C. acetobutylicum to improve the strain's robustness by expressing Escherichia coli's gshAB genes in the adc locus. Acetone production was reduced from 2.64 +/- 0.22 g/L to 0.15 +/- 0.08 g/L in the engineered strain 824adc::gsh, whereas butanol production was increased from 5.17 +/- 0.26 g/L to 8.27 +/- 0.27 g/L. To further improve the alcohol titers, the metabolic flux in the alcohol biosynthesis pathways was enhanced. Overlapping PCR was used to generate expression cassette EC, which expresses the hbd, thl, crt, and bcd genes, and the Sol operon was amplified to express the adhE and ctfAB genes. Butanol and alcohol production reached 14.86 +/- 0.26 g/L and 18.11 +/- 0.66 g/L, respectively, in 824adc:: gsh Sol-EC. Furthermore, the butanol and alcohol yields were 0.336 g/g and 0.409 g/g, respectively, in 824adc:: gsh Sol-EC. This study provided a combined strategy for enhancing alcohol production in C. acetobutylicum. (C) 2013 Elsevier B.V. All rights reserved.
文章类型Article
关键词C. aC.tobutyliC.m Alcohol Production Adc Gene Inactivation Gshab Expression Cassette (Ec) Sol Operon
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiotec.2013.04.013
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]FERMENTATIVE BUTANOL PRODUCTION ; DOWN-REGULATION ; ATCC 824 ; TRANSCRIPTIONAL REGULATION ; MOLECULAR CHARACTERIZATION ; GENE ; DISRUPTION ; EXPRESSION ; BUTYRATE ; THIOLASE
收录类别SCI
语种英语
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000320651500005
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.giec.ac.cn/handle/344007/10131
专题中国科学院广州能源研究所
集成技术研发中心
作者单位1.JiangNan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Hou, Xiaohu,Peng, Wanfeng,Xiong, Lian,et al. Engineering Clostridium acetobutylicum for alcohol production[J]. JOURNAL OF BIOTECHNOLOGY,2013,166(1-2):25-33.
APA Hou, Xiaohu.,Peng, Wanfeng.,Xiong, Lian.,Huang, Chao.,Chen, Xuefang.,...&Zhang, Weiguo.(2013).Engineering Clostridium acetobutylicum for alcohol production.JOURNAL OF BIOTECHNOLOGY,166(1-2),25-33.
MLA Hou, Xiaohu,et al."Engineering Clostridium acetobutylicum for alcohol production".JOURNAL OF BIOTECHNOLOGY 166.1-2(2013):25-33.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hou, Xiaohu]的文章
[Peng, Wanfeng]的文章
[Xiong, Lian]的文章
百度学术
百度学术中相似的文章
[Hou, Xiaohu]的文章
[Peng, Wanfeng]的文章
[Xiong, Lian]的文章
必应学术
必应学术中相似的文章
[Hou, Xiaohu]的文章
[Peng, Wanfeng]的文章
[Xiong, Lian]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。