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Engineering Clostridium acetobutylicum for alcohol production
Hou, Xiaohu1,2; Peng, Wanfeng2; Xiong, Lian2; Huang, Chao2; Chen, Xuefang2; Chen, Xinde2; Zhang, Weiguo1
2013-06-20
Source PublicationJOURNAL OF BIOTECHNOLOGY
Volume166Issue:1-2Pages:25-33
AbstractWhile Clostridium acetobutylicum has been used for large-scale butanol production (ABE fermentation), its by-product acetone cannot be used as a biofuel. In this study, C. acetobutylicum was engineered for alcohol titers (butanol plus ethanol). The adc gene was inactivated to eliminate acetone production, and glutathione biosynthetic capability was introduced into C. acetobutylicum to improve the strain's robustness by expressing Escherichia coli's gshAB genes in the adc locus. Acetone production was reduced from 2.64 +/- 0.22 g/L to 0.15 +/- 0.08 g/L in the engineered strain 824adc::gsh, whereas butanol production was increased from 5.17 +/- 0.26 g/L to 8.27 +/- 0.27 g/L. To further improve the alcohol titers, the metabolic flux in the alcohol biosynthesis pathways was enhanced. Overlapping PCR was used to generate expression cassette EC, which expresses the hbd, thl, crt, and bcd genes, and the Sol operon was amplified to express the adhE and ctfAB genes. Butanol and alcohol production reached 14.86 +/- 0.26 g/L and 18.11 +/- 0.66 g/L, respectively, in 824adc:: gsh Sol-EC. Furthermore, the butanol and alcohol yields were 0.336 g/g and 0.409 g/g, respectively, in 824adc:: gsh Sol-EC. This study provided a combined strategy for enhancing alcohol production in C. acetobutylicum. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordC. aC.tobutyliC.m Alcohol Production Adc Gene Inactivation Gshab Expression Cassette (Ec) Sol Operon
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiotec.2013.04.013
WOS Subject ExtendedBiotechnology & Applied Microbiology
WOS KeywordFERMENTATIVE BUTANOL PRODUCTION ; DOWN-REGULATION ; ATCC 824 ; TRANSCRIPTIONAL REGULATION ; MOLECULAR CHARACTERIZATION ; GENE ; DISRUPTION ; EXPRESSION ; BUTYRATE ; THIOLASE
Indexed BySCI
Language英语
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000320651500005
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.giec.ac.cn/handle/344007/10131
Collection中国科学院广州能源研究所
集成技术研发中心
Affiliation1.JiangNan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Hou, Xiaohu,Peng, Wanfeng,Xiong, Lian,et al. Engineering Clostridium acetobutylicum for alcohol production[J]. JOURNAL OF BIOTECHNOLOGY,2013,166(1-2):25-33.
APA Hou, Xiaohu.,Peng, Wanfeng.,Xiong, Lian.,Huang, Chao.,Chen, Xuefang.,...&Zhang, Weiguo.(2013).Engineering Clostridium acetobutylicum for alcohol production.JOURNAL OF BIOTECHNOLOGY,166(1-2),25-33.
MLA Hou, Xiaohu,et al."Engineering Clostridium acetobutylicum for alcohol production".JOURNAL OF BIOTECHNOLOGY 166.1-2(2013):25-33.
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